反应详情

1.098 g (7.035 mmol) of methyl 3,3,3-trifluoro-2-oxopropanoate in 10 ml of ethanol were initially introduced and heated to reflux. Then, 2.000 g (7.035 mmol) of 1-(2-fluorobenzyl)-1H-pyrazolo[3,4-d]pyrimidine-3-carboximidohydrazide suspended in 25 ml of ethanol were added and the mixture was heated under reflux overnight. After cooling, the mixture was filtered, and the filtercake was washed with a little ethanol and purified by preparative HPLC (mobile phase: acetonitrile/water with 0.1% TFA, ratio 45:55). This gave 710 mg of the target compound (purity 93%; 24% of theory).

1. temperatureheated to reflux
2. additionwere added
3. temperaturethe mixture was heated
4. temperatureunder reflux overnight
5. temperatureAfter cooling
6. filtrationthe mixture was filtered
7. washthe filtercake was washed with a little ethanol
8. custompurified by preparative HPLC (mobile phase: acetonitrile/water with 0.1% TFA, ratio 45:55)