反应详情

To a solution of 2-[(7bR,11aS)-1,2,7b,8,9,10,11,11a-octahydro-4H-pyrido[4,3-b)[1,4]thiazepino[6,5,4-hi]indol-6-yl]-5-methoxybenzaldehyde, the free base of EXAMPLE 16 (0.03 g, 0.08 mmol), in 5 mL of tetrahydrofuran at 0° C. was added methyl magnesium bromide (0.52 mL of a 3M solution in THF, 1.58 mmol). Stirred with warning to ambient temperature for several hours and then quenched with sat'd ammonium chloride. Extracted with ethyl acetate, washed organics with brine, dried (MgSO4) and concentrated. The residue was purified by prep HPLC (C18 reverse phase column, elution with a H2O/CH3CN gradient with 0.5% TFA). Fractions containing desired compound were then immediately free based with 1 N sodium hydroxide, extracted with ethyl acetate, dried (MgSO4) and concentrated to afford the title compound of EXAMPLE 21. LRMS (ES+): 397.1 (M+H)+.

1. waitwith warning to ambient temperature for several hours
2. customquenched with sat'd ammonium chloride
3. extractionExtracted with ethyl acetate
4. washwashed organics with brine
5. dry with materialdried (MgSO4)
6. concentrationconcentrated
7. customThe residue was purified by prep HPLC (
8. washC18 reverse phase column, elution with a H2O/CH3CN gradient with 0.5% TFA)
9. additionFractions containing desired compound
10. extractionextracted with ethyl acetate
11. dry with materialdried (MgSO4)
12. concentrationconcentrated