反应详情

温度

130 °C

A mixture of 0.74 g of methyl 5-(5-methoxy-3H-imidazo[4,5-b]pyridin-3-yl)-3-{[2-(trifluoromethyl)benzyl]oxy}thiophene-2-carboxylate (compound B7b) and 200 ml of a saturated solution of ammonia in methanol was stirred in an autoclave at 130° C. for 10 h. The reaction mixture was allowed to cool down to room temperature and concentrated to about half of the volume. The precipitated solid was filtered, dissolved in 4 ml methanol/DMSO (1:1) and purified by preparative HPLC (water/acetonitrile, elution gradient 9/1 to 1/9 (v/v)). After lyophilization the obtained solid was crystallized with dichloromethane/n-hexane to give the title compound.

1. temperatureto cool down to room temperature
2. concentrationconcentrated to about half of the volume
3. filtrationThe precipitated solid was filtered
4. dissolutiondissolved in 4 ml methanol/DMSO (1:1)
5. custompurified by preparative HPLC (water/acetonitrile, elution gradient 9/1 to 1/9 (v/v))
6. customAfter lyophilization the obtained solid was crystallized with dichloromethane/n-hexane