反应详情

2-{[(2R,6S)-2,6-Dimethyl-4-morpholinyl]methyl}-N-{1-(phenylsulfonyl)-6-[1-(phenylsulfonyl)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1H-indazol-4-yl}-1,3-thiazole-4-carboxamide (99 mg, 0.129 mmol) was suspended in Isopropanol (1 mL) and Sodium hydroxide (1 ml, 2.0 mmol) was added. The mixture was stirred at room temperature for 18 h then neutralised with 2 M HCl (aq.) and the solvent was removed under a stream of nitrogen. The residue was dissolved in DMSO (2 ml) (insoluble salt was filtered off through a filter tube) and the samples (2×1 ml injections) were purified by mass directed autoprep HPLC (Method F). The product-containing fractions were concentrated under a stream of nitrogen to give the title compound as a white solid (18 mg).

1. customthe solvent was removed under a stream of nitrogen
2. dissolutionThe residue was dissolved in DMSO (2 ml) (insoluble salt was filtered off through a filter tube)
3. customthe samples (2×1 ml injections) were purified by mass
4. concentrationThe product-containing fractions were concentrated under a stream of nitrogen