反应详情

5.56 g (10.7 mmol) of [6-hydroxy-2-(4-hydroxyphenyl)benzo[b]thien-3-yl]-[4-[2-(1-piperidinyl)ethoxy]phenyl]methanone was dissolved in 200 ml of dry tetrahydrofuran and 5.45 g (35.2 mmol) of 4-chlorophenyl isocynate was added. The reaction mixture was stirred at room temperature under an atmosphere of nitrogen. After 18 hours, the solvent was removed by evaporation in vacuo, and redissolved in chloroform. The chloroform solution was cooled to −20° C. for 24 hours and the precipitate formed was filtered off. The remaining solution was chromatographed (Waters Prep 500, HPLC) on a silica gel column, eluted with a linear gradient of chloroform ending with chloroform-methanol (19:1)(v/v). The desired fractions were determined by thin layer chromatography, combined and evaporated to dryness to afford 4.01 g of the title compound as a tan amorphous powder.

1. customthe solvent was removed by evaporation in vacuo
2. dissolutionredissolved in chloroform
3. temperatureThe chloroform solution was cooled to −20° C. for 24 hours
4. customthe precipitate formed
5. filtrationwas filtered off
6. customThe remaining solution was chromatographed (Waters Prep 500, HPLC) on a silica gel column
7. washeluted with a linear gradient of chloroform ending with chloroform-methanol (19:1)(v/v)
8. customevaporated to dryness