反应详情

To a stirred solution of (S)-2-t-butoxycarbonylamino-3-biphenyl-4-yl-N-(2-cyanoethyl)-propionamide (20 g, 51.2 mmol) in acetonitrile (220 mL) under nitrogen is added triphenyl phosphine (33.6 g, 128 mmol). The suspension is cooled to 0°. Diisopropyl azodicarboxylate (24.8 mL, 125.6 mmol) is placed in an addition funnel. In a separate addition funnel is placed trimethylsilyl azide (16.8 mL, 127.2 mmol). The two reagents are introduced dropwise, allowing the azoester to be added about 1 minute faster than the silyl azide and keeping the reaction temperature below 10°. The slightly yellow suspension is gradually (10°/hour) warmed up to 35°. The reaction is then monitored by TLC (ethyl acetate/hexane:3/1) until practically complete conversion is observed. A clear solution is then usually obtained. The excess of azide is destroyed by cooling the solution to 10° and adding sodium nitrite (3.2 g) in water (16 mL), followed by acetic acid (16 mL). The mixture is stirred at 20°-25° for at least 2 hours, until a nitrite-free aliquot of the acetonitrile layer shows a negative zide test (ferric chloride paper). The lower salt phase is separated and the organic layer is concentrated in vacuo at 45°. The semi-solid residue is dissolved in isopropanol at 80°. Some insoluble material is filtered from the hot solution. The title compound crystallizes at about 60° after seeding. The suspension is cooled to 25° within 2 hours then left at 0°-5° for 1 hour. The product is filtered, washed with cold ispropanol (3×20 mL) and dried under reduced pressure at 50° to constant weight to obtain (S)-3-[5-(1-t-butoxycarbonylamino-2-biphenyl-4-yl-ethyl)-tetrazol-1-yl]-propionitrile.

1. temperatureThe suspension is cooled to 0°
2. additionIn a separate addition funnel
3. additionThe two reagents are introduced dropwise
4. customthe reaction temperature below 10°
5. temperaturewarmed up to 35°
6. customA clear solution is then usually obtained
7. customThe excess of azide is destroyed
8. temperatureby cooling the solution to 10°
9. additionadding sodium nitrite (3.2 g) in water (16 mL)
10. customThe lower salt phase is separated
11. concentrationthe organic layer is concentrated in vacuo at 45°
12. dissolutionThe semi-solid residue is dissolved in isopropanol at 80°
13. filtrationSome insoluble material is filtered from the hot solution
14. customThe title compound crystallizes at about 60° after seeding
15. temperatureThe suspension is cooled to 25° within 2 hours
16. filtrationThe product is filtered
17. washwashed with cold ispropanol (3×20 mL)
18. customdried under reduced pressure at 50° to constant weight