反应详情

To a solution of (S)-tert-butyl 7-((3R,5S)-1-((S)-2-(((benzyloxy)carbonyl)amino)-3,3-dimethylbutanoyl)-5-(((R)-1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)pyrrolidin-3-yl)-3-(((R)-1,2,3,4-tetrahydronaphthalen-1-yl)carbamoyl)-3,4-dihydroisoquinoline-2(1H)-carboxylate (0.44 g, 0.49 mmol) in DCE (5 mL) was added TFA (1.2 mL, 15.6 mmol). The reaction mixture was stirred at rt for 2 h and concentrated in vacuo. The residue was basified with sat. aq. NaHCO3 solution. The resulting mixture was extracted with EtOAc (2×) and the organic layer was separated, washed with brine and dried over MgSO4. The filtrate was concentrated in vacuo to give the title compound as a light yellow solid (0.35 g, 91%). MS (ESI+) m/z 796.5 (M+H)+.

1. concentrationconcentrated in vacuo
2. extractionThe resulting mixture was extracted with EtOAc (2×)
3. customthe organic layer was separated
4. washwashed with brine
5. dry with materialdried over MgSO4
6. concentrationThe filtrate was concentrated in vacuo