反应详情

The compound obtained in the Example 1 (150 mg) was suspended in DMF (30 ml), and 1-hydroxybenzotriazole (74.4 mg), 1-(dimethylaminopropyl)-3-ethylcarbodiimide (106 mg), and a 2 M-ammonia/ethanol solution (0.92 ml) was added to the suspension at room temperature. After the mixture was stirred overnight, chloroform and saturated sodium bicarbonate aqueous solution were added to terminate the reaction. After the aqueous layer was extracted with chloroform twice, the combined organic layer was washed with saturated brine. After the organic layer was dried over anhydrous magnesium sulfate, the solvent was evaporated under reduced pressure. The resulting residue was purified on column chromatography (MeOH:CHCl3=1:10) to obtain the title compound (128 mg) as a pale yellow solid.

1. customto terminate
2. customthe reaction
3. extractionAfter the aqueous layer was extracted with chloroform twice
4. washthe combined organic layer was washed with saturated brine
5. dry with materialAfter the organic layer was dried over anhydrous magnesium sulfate
6. customthe solvent was evaporated under reduced pressure
7. customThe resulting residue was purified on column chromatography (MeOH:CHCl3=1:10)