反应详情

温度

150 °C

4-[5-(Chloromethyl)-1,3,4-oxadiazol-2-yl]-6-(1H-indol-4-yl)-1-(phenylsulfonyl)-1H-indazole (50 mg, 0.102 mmol) and sodium methanesulfinate (10.42 mg, 0.102 mmol) in ethanol (0.8 ml) were heated under microwave irradiation to 100° C. for 30 mins then 150° C. for 30 min, followed by 150° C. for a further 30 mins. Additional sodium methanesulfinate (10.42 mg, 0.102 mmol) was added and the reaction heated to 150° C. for a further 30 mins. The solvent was removed and the resultant beige solid treated with isopropanol (1 ml) and 2M aqueous sodium hydroxide (1.003 ml, 2.006 mmol) and stirred at 20° C. for 20 h. The mixture was neutralised with 2M aqueous hydrogen chloride, evaporated to dryness and azeotroped with isopropanol (2 ml). The residue was partitioned between dichloromethane (5 ml) and water (5 ml) and shaken vigorously. The aqueous phase was pipetted off, then the heterogeneous solution was treated with methanol (5 ml), absorbed onto Florisil and the solvents removed. The Florisil was placed on top of a silica cartridge (10 g) and eluted with 0-3% methanol/dichloromethane. Appropriate fractions were combined and concentrated to give a beige solid, 16 mg. Further purification by Mass Directed Automated Preparative HPLC afforded the title compound as a white solid (5 mg).

1. waitfollowed by 150° C. for a further 30 mins
2. customThe solvent was removed
3. customevaporated to dryness
4. customazeotroped with isopropanol (2 ml)
5. customThe residue was partitioned between dichloromethane (5 ml) and water (5 ml)
6. stirringshaken vigorously
7. additionthe heterogeneous solution was treated with methanol (5 ml)
8. customabsorbed onto Florisil
9. customthe solvents removed
10. washeluted with 0-3% methanol/dichloromethane
11. concentrationconcentrated
12. customto give a beige solid, 16 mg
13. customFurther purification by Mass Directed Automated Preparative HPLC