反应详情

温度

50 °C

To a slurry of 3-(benzyloxy)-9,9-dimethyl-4-oxo-4,6,7,9-tetrahydropyrimido[2,1-c][1,4]oxazine-2-carbothioamide (0.04 g, 0.116 mmol, 1.0 equiv) in acetonitrile (1.2 mL) under a nitrogen atmosphere, was added MeI (0.011 mL, 0.174 mmol, 1.5 equiv). The reaction was heated at 50° C. (oil bath) for 1 h. The reaction was then cooled to ambient temperature and concentrated in vacuo. 2-(4-Fluorophenyl)acetohydrazide (0.058 g, 0.35 mmol, 3 equiv) was then added to the residue followed by methanol (1 mL) and the slurry was heated at 70° C. (oil bath). After stirring 1 h, the reaction was cooled to ambient temperature and concentrated in vacuo. DMF (1 mL) was added to the residue and the solution was heated at 150° C. (oil bath) for 3.5 h. The reaction was then cooled to ambient temperature and stirred 3 d. The reaction was heated at 140° C. (oil bath) for 2 h. The reaction was cooled to ambient temperature and concentrated in vacuo. The residue was added to a saturated aqueous solution of NaHCO3 and extracted with CH2Cl2 (×2). The combined CH2Cl2 extracts were dried (Na2SO4) and then concentrated in vacuo. The crude product was purified by reverse phase preparatory HPLC to provide the title compound (0.021 g, 49% yield) as a gray solid. 1H NMR (400 MHz, CDCl3) δ ppm 7.99 (s, 2H), 7.21-7.39 (m, 2H), 7.00 (t, J=8.69 Hz, 2H), 4.17 (s, 2H), 4.05 (s, 4H), 1.62 (s, 6H); LCMS (ES+, (M+H)+) m/z 372.16.

1. temperatureThe reaction was then cooled to ambient temperature
2. concentrationconcentrated in vacuo
3. temperaturethe slurry was heated at 70° C. (oil bath)
4. temperaturethe reaction was cooled to ambient temperature
5. concentrationconcentrated in vacuo
6. additionDMF (1 mL) was added to the residue
7. temperaturethe solution was heated at 150° C. (oil bath) for 3.5 h
8. temperatureThe reaction was then cooled to ambient temperature
9. stirringstirred 3 d
10. temperatureThe reaction was heated at 140° C. (oil bath) for 2 h
11. temperatureThe reaction was cooled to ambient temperature
12. concentrationconcentrated in vacuo
13. additionThe residue was added to a saturated aqueous solution of NaHCO3
14. extractionextracted with CH2Cl2 (×2)
15. dry with materialThe combined CH2Cl2 extracts were dried (Na2SO4)
16. concentrationconcentrated in vacuo
17. customThe crude product was purified by reverse phase preparatory HPLC