反应详情

A mixture of 4-(2,3-diaminobenzoyl)amino-3-methoxy-N-methyl-N-[4-methyl-2-[5-(4-methylpiperazin-1-yl)carbonylpent-1-yloxy]phenyl]benzamide (90 mg) and trimethyl orthoformate (1 ml) was refluxed for 4 hours. After removing excess reagent by evaporation, the residue was dissolved in chloroform and the solution was washed with water and saturated aqueous sodium bicarbonate solution. The organic layer was dried over magnesium sulfate and the solvent was evaporated in vacuo. The residue was purified by preparative thin-layer chromatography (chloroform-methanol=10:1) to give 4-(1H-benzimidazol-4-yl)carbonylamino-3-methoxy-N-methyl-N-[4-methyl-2-[5-(4-methylpiperazin-1-yl)carbonylpent-1-yloxy]phenyl]benzamide (51 mg) as a powder.

1. temperaturewas refluxed for 4 hours
2. customAfter removing excess reagent
3. customby evaporation
4. dissolutionthe residue was dissolved in chloroform
5. washthe solution was washed with water and saturated aqueous sodium bicarbonate solution
6. dry with materialThe organic layer was dried over magnesium sulfate
7. customthe solvent was evaporated in vacuo
8. customThe residue was purified by preparative thin-layer chromatography (chloroform-methanol=10:1)