反应详情

(2S,4S)-tert-butyl 4-(4-amino-3-((3,5-dimethoxyphenyl)ethynyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)-2-((tert-butyldiphenylsilyloxy)methyl)pyrrolidine-1-carboxylate (25 mg) obtained in Step 4 above was dissolved in THF (1.0 ml). Silica gel-supported tetrabutylammonium fluoride (upto 1.5 mmol/g, 34 mg) was added thereto, followed by stirring overnight. Silica gel-supported tetrabutylammonium fluoride (upto 1.5 mmol/g, 30 mg) was further added thereto, and the mixture was further stirred for 2 days. After filtering the reagent off, the solvent was distilled off under reduced pressure. The resulting residue was purified by basic silica gel column chromatography (developing solvent: hexane/ethyl acetate) to obtain the title compound as a colorless, amorphous substance (62 mg). Physical properties: m/z [M+H]+ 495.1

1. additionSilica gel-supported tetrabutylammonium fluoride (upto 1.5 mmol/g, 34 mg) was added
2. additionwas further added
3. stirringthe mixture was further stirred for 2 days
4. filtrationAfter filtering the reagent off, the solvent
5. distillationwas distilled off under reduced pressure
6. customThe resulting residue was purified by basic silica gel column chromatography (developing solvent: hexane/ethyl acetate)