反应详情

温度

60 °C

A mixture of 3-bromo-1-[1-(4-fluorophenyl)-5-isopropylpyrazol-4-yl]pyrrolidin-2-one (0.055 g, 0.15 mmol), 4-amino-1H-pyrazolo[3,4-d]pyrimidine-3-carbonitrile (0.050 g, 0.31 mmol) and K2CO3 (0.050 g, 0.36 mmol) in DMF (2.0 mL) was stirred at 60° C. for 1 hr. The mixture was then cooled to room temperature, quenched with water (30 mL), and extracted with IPA:CHCl3 (1:2 v/v, 100 mL). The organic layer was separated, dried over anhydrous sodium sulfate, concentrated in vacuo and purified by flash chromatography (SiO2, 0˜10% MeOH/EtOAc gradient elution) to give 4-amino-1-[1-[1-(4-fluorophenyl)-5-isopropylpyrazol-4-yl]-2-oxo-pyrrolidin-3-yl]pyrazolo[3,4-d]pyrimidine-3-carbonitrile (0.055 g, 82%).

1. temperatureThe mixture was then cooled to room temperature
2. customquenched with water (30 mL)
3. extractionextracted with IPA:CHCl3 (1:2 v/v, 100 mL)
4. customThe organic layer was separated
5. dry with materialdried over anhydrous sodium sulfate
6. concentrationconcentrated in vacuo
7. custompurified by flash chromatography (SiO2, 0˜10% MeOH/EtOAc gradient elution)