反应详情

On the other hand, the filtrate and the washing liquid were well washed with chlorobenzene (5 ml) and then combined with each other, thereby a clear solution was obtained. When 10% aqueous sodium carbonate solution (12 ml) was added to the solution, a solid was deposited. After the mixture was allowed to mature by stirring at room temperature for 1 hour and then under ice-cooling for 1 hour, the deposited solid was filtered, and sequentially washed with chlorobenzene (2 ml) and water (2 ml), and dried under vacuum, thereby a white solid was obtained. The purity of the target Tyr(Bn)-Gly-Gly-Phe-Leu-OBn (SEQ ID NO: 2) was 88%, and the content of Tyr(Bn) was 4.9%. Further, the crystallization yield was 88%, and the acquisition yield relative to the Leu-OBn.TsOH salt was 58%.

1. washOn the other hand, the filtrate and the washing liquid were well washed with chlorobenzene (5 ml)
2. customwas obtained
3. temperatureunder ice-cooling for 1 hour
4. filtrationthe deposited solid was filtered
5. washsequentially washed with chlorobenzene (2 ml) and water (2 ml)
6. customdried under vacuum
7. customa white solid was obtained
8. customFurther, the crystallization yield was 88%