反应详情

The microbial cells of Serratia marcescens TA5002 obtained by the same manner as in Example 3 (10 g) was suspended in 0.05M phosphate buffer (pH 8.5, 50 ml) and sonicated at 9 Kc for 10 minutes. Then, the suspension was centrifuged and the resulting supernatant (35 ml) was passed through a column packed with Duolite-A7 (a weak basic anion exchange resin manufactured by Diamond Shamrock Chemical Co. in U.S.A.) (60 ml) at room temperature at SV=0.75. Then, the column was subjected to cross-linking treatment with a solution containing 0.1M phosphate buffer (pH 8.5, 300 ml) and 0.4% glutaraldehyde (300 ml) for 30 minutes and excess glutaraldehyde was thoroughly washed out to give an immobilized enzyme. The immobilized enzyme was packed in a column (volume 50 ml) and 1M ammonium fumarate solution containing 1mM magnesium chloride (pH 8.5, 500 ml) was passed through the column at the flow rate of 25 ml/hr. The combined effluent was adjusted to pH 2.8 to precipitate crystals. The crystals were filtered off to give L-aspartic acid (58 g).

1. customsonicated at 9 Kc for 10 minutes
2. customat room temperature
3. washexcess glutaraldehyde was thoroughly washed out
4. customto give an immobilized enzyme
5. customto precipitate crystals
6. filtrationThe crystals were filtered off