反应详情

A suspension of 3-amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (Int. A) (200 mg, 0.702 mmol) in NMP (6 ml) was treated with (R)-benzyl 2-hydrazinyl-2-oxo-1-phenylethylcarbamate (Int. JR) (231 mg, 0.772 mg) followed by portionwise addition of HATU (293 mg, 0.772 mmol). After stirring at RT for 45 minutes, the reaction mixture was added to EtOAc (50 ml) and washed with 0.1 M NaOH (50 ml). The aqueous layer was back extracted with EtOAc (25 ml). The combined organic portions were washed with water (75 ml), brine (50 ml), dried (MgSO4) and concentrated in vacuo. The crude product was loaded in MeOH onto a pre-wetted (MeOH) Isolute® PE-AX (anion exchange) cartridge. The cartridge was eluted with MeOH (70 ml) and the filtrate was concentrated in vacuo to give a yellow solid which was placed under high vacuum to give the title compound as a yellow solid;

1. washwashed with 0.1 M NaOH (50 ml)
2. extractionThe aqueous layer was back extracted with EtOAc (25 ml)
3. washThe combined organic portions were washed with water (75 ml), brine (50 ml)
4. dry with materialdried (MgSO4)
5. concentrationconcentrated in vacuo
6. washThe cartridge was eluted with MeOH (70 ml)
7. concentrationthe filtrate was concentrated in vacuo
8. customto give a yellow solid which