反应详情

4-[6-(8-Cyclopentyl-6-fluoro-7-oxo-7,8-dihydro-pyrido[2,3-d]pyrimidin-2-ylamino)-pyridin-3-yl]-piperazine-1-carboxylic acid tert-butyl ester (0.195 g, 0.38 mmol) prepared as in Example 17, was dissolved in 1:1 chloroform/methanol (8 ml), purged with anhydrous hydrogen chloride gas and stirred for 2.5 hours at room temperature. To the mixture was added diethyl ether (15 ml) giving a precipitate that was filtered, washed with ether and dried in vacuo to provide 8-cyclopentyl-6-fluoro-2-(5-piperazin-1-yl-pyridin-2-ylamino)-8H-pyrido[2,3-d]pyrimidin-7-one hydrochloride as a yellow solid (0.177 g, 88%). MS (APCI); M++1: Calc, 410.46. Found, 410.3. Analyses for C21H24N7O.1.0 H2O.2.0HCl: Calc'd: C, 50.73; H, 5.75; N, 19.46; Cl (ionic), 13.77; H2O, 1.41. Found: C, 50.41; H, 5.64; N, 19.59; Cl (ionic), 14.16; H2O, 3.60.

1. custompurged with anhydrous hydrogen chloride gas
2. additionTo the mixture was added diethyl ether (15 ml)
3. customgiving a precipitate that
4. filtrationwas filtered
5. washwashed with ether
6. customdried in vacuo