反应详情

Peptides were synthesised using a CS-Bio 336S, a CS-Bio 336X and a Multipep peptide synthesis machine (Intavis AG Bioanalytical Instruments, Germany). Standard amino acids were purchased from CS-Bio in their Fmoc-N-α-protected form. Fmoc-trans-4-hydroxy-(L)-proline was obtained from Merck. Fmoc-cis-4-hydroxy-(L)-proline, Fmoc-cis-3-hydroxy-(L)-proline and Fmoc-trans-3-hydroxy-(L)-proline were prepared as described in the synthetic section. Peptide synthesis was carried out in dimethylformamide (DMF) using diisopropylcarbodiimide (DIC) and 1-hydroxybenzotriazole (HOBt) as activating reagents, and piperidine as Fmoc deprotection reagent. DIC was used as 0.5 M solution in DMSO, and HOBt as a 0.5 M solution in DMF. Typically, synthesis was carried out on a 0.1 mmol scale, using MBHA Rink Amide resin (loading 0.45 mmol/g), 1 mmol of protected amino acids (10× molar excess), and 1 mmol of activating reagents (DIC, HOBt). Parallel peptide synthesis for screening was performed on Tentagel S-RAM resin. Amino acids were pre-activated by addition of DIC/HOBt for 30 min prior to addition to the resin. After addition to the resin, the activated amino acid was allowed to react with the resin/growing peptide chain for 2 h, prior to removal and washing of the resin. Fmoc deprotection was performed using 20% piperidine, 20% DMSO in 60% DMF, followed by extensive washing of the resin, and addition of the next amino acid.

1. customTypically, synthesis
2. additionto addition to the resin
3. additionAfter addition to the resin
4. customto react with the resin/growing peptide chain for 2 h
5. customto removal
6. washwashing of the resin
7. washby extensive washing of the resin, and addition of the next amino acid