反应详情

A suspension of the phenylmethyl ester product from part (g) (0.8 g., 1.5 mmole) in methanol and palladium hydroxide (20% on carbon) is hydrogenated at room temperature and atmospheric pressure for 30 minutes. The mixture is filtered, the solids are rinsed with methanol, and the filtrate is concentrated to give 0.42 g. of yellow oil. The residue is chromatographed on LPS-1 silica gel using an elution gradient of 3→10% acetic acid in chloroform. Fractions containing the desired material are combined and concentrated. The residue is dissolved in methanol/water, filtered, concentrated (methanol removed) and lyophilized to give a white solid. The material is rechromatographed on CC-4 silica gel using ethyl acetate as the eluant. Fractions containing the desired produced are combined and lyophilized from water/dioxane to give 1-[[methyl[(S)-2-oxo-4-phenyl-3-[(1-piperidinylcarbonyl)amino]butyl]amino]carbonyl]-L-proline as a white solid; m.p. 69°-72°; [α]D25 =-37° (c=0.2, methanol). TLC (silica gel; toluene:acetic acid, 4:1) Rf =0.18.

1. customis hydrogenated at room temperature
2. filtrationThe mixture is filtered
3. washthe solids are rinsed with methanol
4. concentrationthe filtrate is concentrated
5. customto give 0.42 g
6. customThe residue is chromatographed on LPS-1 silica gel using an elution gradient of 3→10% acetic acid in chloroform
7. additionFractions containing the desired material
8. concentrationconcentrated
9. dissolutionThe residue is dissolved in methanol/water
10. filtrationfiltered
11. concentrationconcentrated
12. custom(methanol removed)
13. customto give a white solid
14. customThe material is rechromatographed on CC-4 silica gel
15. additionFractions containing the
16. customdesired produced