反应详情

A solution of 2,2,2-trichloro-1-{10-[(4-chlorophenoxy)acetyl]-10,11-dihydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-3-yl}ethanone of Example 67 (0.3 mmol) and tyramine (0.62 mmol) in dimethylsulfoxide (0.2 mL) and acetonitrile (4 mL) was stirred at 80° C. for 18 hours. The solvents were evaporated and the residue was dissolved in dichloromethane, washed with water, dried over anhydrous sodium sulfate and evaporated. The compound was purified by HPLC (normal phase, Luna® CN bonded packing) and crystallized from ethyl acetate/hexane (0.12 g), m.p. 206-207 ° C. MS [(+)ESI, m/z]: 516 [M+H]+ Anal. Calcd for C29H26CIN3O4: C, 67.50; H, 5.08; N, 8.14. Found: C, 67.35; H, 5.07; N, 8.08.

1. customThe solvents were evaporated
2. dissolutionthe residue was dissolved in dichloromethane
3. washwashed with water
4. dry with materialdried over anhydrous sodium sulfate
5. customevaporated
6. customThe compound was purified by HPLC (normal phase, Luna® CN bonded packing)
7. customcrystallized from ethyl acetate/hexane (0.12 g), m.p. 206-207 ° C