反应详情

温度

100 °C

A solution of 2-methyl-2-fluoropropionic acid (108 mg, 1.02 mmol) and 1′1′-carbonyldiimidazole (144 mg, 0.888 mmol) in anhydrous DMF (2.5 ml) was stirred at room temperature under nitrogen atmosphere for 30 min. To this was added N′-Hydroxy-4-{4-methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazol-3-yl}bicyclo[2.2.2]octane-1-carboximidamide (1-G) (139.5 mg, 0.355 mmol) and the solution stirred overnight under N2. The reaction was heated for 1.5 hr at 100° C. in a heat block. DMF was removed in vacuo and the solid was redissolved in CH3CN (4 ml). The product was purified by C-18 reverse phase chromatography eluting with 10-90% CH3CN (0.1% TFA)/water (0.1% TFA). The solvent was removed and the residue taken up in DCM and free-based from saturated aqueous sodium bicarbonate solution. The organic layers were dried over MgSO4 and filtered. The solvent was replaced with CH3CN/water and lyophilized to afford 5-(1-fluoro-1-methylethyl)-3-(4-{4-methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazol-3-yl}bicyclo[2.2.2]oct-1-yl)-1,2,4-oxadiazole (3-A) as a white solid. MS (ESI+)=464.13 (M+1). 1H NMR (500 MHz, CDCl3): δ 7.89-7.85 (m, 1H), 7.75-7.69 (m, 2H), 7.55 (t, 1H), 3.52 (s, 3H), 2.30 (dd, 6H), 2.15 (dd, 6H), 1.90 (s, 3H), 1.86 (s, 3H). Shh-Light II Assay: IC50: 4.2 μM.

1. customDMF was removed in vacuo
2. dissolutionthe solid was redissolved in CH3CN (4 ml)
3. customThe product was purified by C-18 reverse phase chromatography
4. washeluting with 10-90% CH3CN (0.1% TFA)/water (0.1% TFA)
5. customThe solvent was removed
6. dry with materialThe organic layers were dried over MgSO4
7. filtrationfiltered