反应详情

温度

0 °C

To a solution of ethyl 3-cyclopropyl-3-(6-((6-(2-fluoro-5-methoxyphenyl)-5-(3,3,3-trifluoropropoxy) pyridin-3-yl)methoxy)pyrimidin-4-yl)propanoate (53 mg) in methanol (1.0 mL) was added 2N aqueous sodium hydroxide (235 μL) solution at room temperature, and the mixture was stirred for 20 hr. The reaction mixture was cooled to 0° C., neutralized with 1N hydrochloric acid and extracted with ethyl acetate. The extract was washed with saturated brine. The organic layer was dried over anhydrous sodium sulfate, and the solvent was evaporated under reduced pressure. The residue was purified by preparative HPLC (C18, mobile phase: water/acetonitrile (10 mM NH4HCO3-containing system)), and the obtained fraction was evaporated under reduced pressure to give the title compound (32 mg) as a colorless oil.

1. extractionextracted with ethyl acetate
2. washThe extract was washed with saturated brine
3. dry with materialThe organic layer was dried over anhydrous sodium sulfate
4. customthe solvent was evaporated under reduced pressure
5. customThe residue was purified by preparative HPLC (C18, mobile phase: water/acetonitrile (10 mM NH4HCO3-containing system))
6. customthe obtained fraction was evaporated under reduced pressure