反应详情

To a stirred solution of 5-(4-Chlorophenyl)-1-(4-(N-((dimethylamino)methylene) sulfamoyl)phenyl)-N-methoxy-N,2,4-trimethyl-1H-pyrrole-3-carboxamide (step 3, 0.750 g, 1.49 mmol) in anhydrous THF (50 ml) at 0° C., ethyl magnesium bromide (Grignard reagent, 0.994 g, 7.4 ml, 7.46 mmol) was added dropwise and reaction mixture was heated to reflux for 1 h. The completion of reaction was monitored by TLC. After cooling, reaction mixture was quenched by addition of solution of saturated ammonium chloride (10 ml) and extracted with ethyl acetate (1×50 ml). Combined organic layer was dried over anhydrous Na2SO4. The solvent was evaporated under reduced pressure to obtain a crude product; which was purified by column chromatography over silica gel (100-200 mesh) using 0.4% methanol in dichloromethane as an eluent to yield the title compound which was finally purified by preparative HPLC (0.062 g, 10%)

1. customreaction mixture
2. temperaturewas heated
3. temperatureto reflux for 1 h
4. customThe completion of reaction
5. temperatureAfter cooling
6. customreaction mixture
7. customwas quenched by addition of solution of saturated ammonium chloride (10 ml)
8. extractionextracted with ethyl acetate (1×50 ml)
9. dry with materialCombined organic layer was dried over anhydrous Na2SO4
10. customThe solvent was evaporated under reduced pressure
11. customto obtain a crude product
12. customwhich was purified by column chromatography over silica gel (100-200 mesh)