反应详情

温度

50 °C

A solution of tetrabutylammonium fluoride (1M in THF, 1 mL, 1 mmol) was added to a solution of {6-amino-3′-[5-(tert-butoxycarbonylamino-imino-methyl)-2-methylsulfanyl-thiophene-3-sulfonyl]-2-methyl-biphenyl-4-yl}-carbamic acid 2-trimethylsilanyl-ethyl ester ((Example 294: step f) 86 mg, 0.11 mmol) in THF (1 mL). The solution was heated at 50° C. for 12 h, then the solution was partitioned between EtOAc (50 mL) and water (20 mL). The layers were separated and the organic layer was further extracted with water (5×10 mL) and brine (20 mL). The solution was dried over sodium sulfate and concentrated in vacuo to yield the title compound (71 mg, 84%) which was used without further purification. ESI-MS (m/z): Calcd. for C24H28N4O4S3 (M+H): 533.1; found: 532.7.

1. customthe solution was partitioned between EtOAc (50 mL) and water (20 mL)
2. customThe layers were separated
3. extractionthe organic layer was further extracted with water (5×10 mL) and brine (20 mL)
4. dry with materialThe solution was dried over sodium sulfate
5. concentrationconcentrated in vacuo