反应详情

[5-Amino-1-(2-methyl-1H-benzimidazol-5-yl)-1H-pyrazol-4-yl]-[3-fluoro-1-(toluene-4-sulfonyl)-1H-indol-2-yl]-methanone (25.0 mg, 0.041 mmol) was dissolved in methane sulfonic acid (0.25 ml), and stirred at room temperature for one hour. The pH of the reaction mixture was adjusted to 6 using an aqueous solution of 1 M sodium hydroxide. After extraction with ethyl acetate, the extract was dried over anhydrous sodium sulfate. The desiccant was removed by filtration. The filtrate was concentrated under reduced pressure, and the resulting residue was purified by silica gel column chromatography (dichloromethane/methanol=60/1 to 40/1) to give [5-amino-1-(2-methyl-1H-benzimidazol-5-yl)-1H-pyrazol-4-yl]-(3-fluoro-1H-indol-2-yl)-methanone was obtained as a pale yellow solid (8.7 g, with a yield of 55%).

1. extractionAfter extraction with ethyl acetate
2. dry with materialthe extract was dried over anhydrous sodium sulfate
3. customThe desiccant was removed by filtration
4. concentrationThe filtrate was concentrated under reduced pressure
5. customthe resulting residue was purified by silica gel column chromatography (dichloromethane/methanol=60/1 to 40/1)