反应详情

温度

60 °C

The methyl 2-hydroxy-5-(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenamidobenzoate was then dissolved in THF (15 mL) and 3N NaOH (5 mL). The mixture was heated (60° C., 2 h) and then concentrated and acidified to pH 3 with 2N HCl. The product was extracted with ethyl acetate, and the combined organic extracts were washed with brine and dried over MgSO4. The crude product was purified by silica gel chromatography (0-15% MeOH/CH2Cl2) to afford 2-hydroxy-5-(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenamidobenzoic acid (0.3 g) as an orange solid. Mass calculated for C27H37NO4=439.59; found: [M−H]+=438.3.

1. concentrationconcentrated
2. extractionThe product was extracted with ethyl acetate
3. washthe combined organic extracts were washed with brine
4. dry with materialdried over MgSO4
5. customThe crude product was purified by silica gel chromatography (0-15% MeOH/CH2Cl2)