N-乙酰基L-甲状腺素 → Thyroxine

HRID 1388087

反应方程式

HRID 1388087 反应方程式
C([NH:4][C@H:5]([C:25]([OH:27])=[O:26])[CH2:6][C:7]1[CH:14]=[C:12]([I:13])[C:11]([O:15][C:16]2[CH:23]=[C:21]([I:22])[C:20]([OH:24])=[C:18]([I:19])[CH:17]=2)=[C:9]([I:10])[CH:8]=1)(=O)C>B([O-])([O-])[O-].CN(C=O)C>[NH2:4][C@H:5]([C:25]([OH:27])=[O:26])[CH2:6][C:7]1[CH:8]=[C:9]([I:10])[C:11]([O:15][C:16]2[CH:17]=[C:18]([I:19])[C:20]([OH:24])=[C:21]([I:22])[CH:23]=2)=[C:12]([I:13])[CH:14]=1

参与物

4

反应物 1

HCID 20638380N-乙酰基L-甲状腺素

产物 1

HCID 5819Thyroxine

试剂、催化剂与溶剂 2

HCID 6228N,N-二甲基甲酰胺溶剂2 次HCID 26574Borate溶剂

实验过程

STI is dissolved in 50 ml of borate-saline buffer pH 8.5, to a concentration of 16 mg/ml (38 μmole). N-hydroxysuccinimide ester of N-acetyl thyroxine (378 μmole, 346 mg) is dissolved in 50 ml of DMF in a separate container. Aliquots (2 ml) of the active ester in DMF are added to the STI at 5 minute intervals until all the active ester has been added. The reaction mixture is stirred at room temperature for an additional 3.5 hours, then terminated by dialysis against 2 liters of aqueous triethylamine pH 11. Chromatography of the product on a Sephadex G-75 column (5×100 cm) equilibrated with aqueous triethylamine, pH 11 is then conducted to isolate thyroxine (T4) conjugated to soybean trypsin inhibitor from free T4. Fractions containing 1-2 molecules of thyroxine per molecule of protein are isolated as the preferred reagent. Higher substituted fractions were obtained, although operable, they were less preferable.

后处理

  1. additionhas been added
  2. customterminated by dialysis against 2 liters of aqueous triethylamine pH 11

来源与证据

ORD记录
ord-afae8acb7d9b4e64846b696f9c55c639
来源数据集
uspto-grants-1984_02
专利号
US04430263

关联笔记

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