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氯化钾 → 2,5-Dihydrofuran-3,4-dicarboxylic acid
参与物
| 角色 | 名称 | HCID | 投料 |
|---|---|---|---|
| 反应物 | 氯化钾 | HCID 4873 | — |
| 反应物 | 2,6-二氯靛酚 | HCID 13726 | — |
| 反应物 | X 100 | HCID 19153 | — |
| 反应物 | Dihydroorotate | HCID 5461056 | — |
| 溶剂 | 三羟甲基氨基甲烷盐酸盐 | HCID 93573 | — |
| 产物 | 2,5-Dihydrofuran-3,4-dicarboxylic acid | HCID 6420982 | — |
反应步骤
The standard assay mixture contained 50 μM decyclo ubichinone, 100 μM dihydroorotate, 60 μM 2,6-dichloroindophenol, as well as 20 mU DHODH. The volume activity of the recombinant enzyme used was 30 U/ml. Measurements were conducted in 50 mM TrisHCl (150 mM KCl, 0,1% Triton X-100, pH. 8,0) at 30° C. in a final volume of 1 ml. The components were mixed, and the reaction was started by adding dihydroorotate. The course of reaction was followed by spectrophotometrically measuring the decrease in absorption at 600 nm for 2 min.
来源与证据
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 专利号 | US07423057B2 |
| 来源数据集 | uspto-grants-2008_09 |
| ORD ID | ord-8c87408e6e1e4db5a86984764b0046b0 |
| 来源说明 | uspto-grants-2008_09 |