三乙酸甘油酯 → Hydrogen Peroxide

HRID 384562

反应方程式

HRID 384562 反应方程式
[CH3:1][C:2](=[O:15])[O:3][CH2:4][CH:5]([CH2:10][O:11][C:12](=[O:14])[CH3:13])[O:6][C:7](=[O:9])[CH3:8].C([O-])([O-])=O.C([O-])([O-])=O.OO.OO.OO.[Na+].[Na+].[Na+].[Na+].C([O:37][OH:38])(=O)C>>[CH3:13][C:12](=[O:14])[O:11][CH2:10][CH:5]([CH2:4][O:3][C:2](=[O:15])[CH3:1])[O:6][C:7](=[O:9])[CH3:8].[OH:37][OH:38]

参与物

5

反应物 3

HCID 5541三乙酸甘油酯HCID 6585过氧乙酸HCID 159762过碳酸钠

产物 2

HCID 784Hydrogen PeroxideHCID 5541三乙酸甘油酯

实验过程

The procedures described in Example 44 and Example 45 were repeated using total protein from a heat-treated, centrifuged cell extract supernatant from a transformant expressing perhydrolase (SEQ ID NO. 82) from Thermotoga lettingae (KLP18/pSW220, Example 42) or a transformant expressing perhydrolase (SEQ ID NO. 90) from Thermotoga petrophila (KLP18/pSW222, Example 43), except that sodium percarbonate (˜25 wt % H2O2) was substituted for aqueous hydrogen peroxide to produce an initial concentration of either 100 mM or 250 mM hydrogen peroxide. Reactions containing triacetin, sodium percarbonate and heat-treated, centrifuged cell extract supernatant in 50 mM sodium bicarbonate buffer (2 mL total volume, pH 8.1) were run at 24° C. A control reaction for each reaction condition was run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added extract protein. The concentration of peracetic acid in the reaction mixtures was determined according to the method of Karst et al. described in Example 2. The peracetic acid concentrations produced in 1 min, 5 min and 30 min are listed in Table 31.

后处理

  1. additionheat-treated
  2. extractionextract supernatant in 50 mM sodium bicarbonate buffer (2 mL total volume, pH 8.1)
  3. customwere run at 24° C
  4. customA control reaction for each reaction condition

来源与证据

ORD记录
ord-a12c0aaab47943cfb11a334e09132923
来源数据集
uspto-grants-2013_02
专利号
US08367728B2

关联笔记

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