L-Leucine → L-酪氨酸聚合物

HRID 395256

反应方程式

HRID 395256 反应方程式
[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH2:3][CH:4]([CH3:6])[CH3:5].C[CH2:11][CH:12]([OH:17])[C:13](OC)=O.CCC(O)C(OC)=O.N[C@H](C(O)=O)CC(C)C.C(OC(=O)[C@H](CC(C)C)N)C.N[C@H](C(O)=O)C[CH2:49][S:50]C.CCC(O)C(OC)=O.N[C@H](C(O)=O)CCSC>>[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH2:3][CH2:4][S:50][CH3:49].[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH2:3][C:4]1[CH:6]=[CH:13][C:12]([OH:17])=[CH:11][CH:5]=1

参与物

8

反应物 6

HCID 6106L-Leucine2 次HCID 6137L-MethionineHCID 75971L-亮氨酸乙酯HCID 520445Methyl 2-hydroxybutanoateHCID 131724020HCID 131724020，化合物HCID 131724021HCID 131724021，化合物

产物 2

HCID 6057L-酪氨酸聚合物HCID 6137L-Methionine

实验过程

The papain-catalyzed synthesis of leucine and MHBA co-oligomers was also explored. Synthesis of leucine oligomers and MHBA-leucine co-oligomers was initiated with leucine ethyl ester and MHBA ethyl ester as the substrates. The overall synthesis and purification approach was similar to the one used in the case of methionine and MHBA-methionine. Reaction rates similar to those obtained with methionine and tyrosine were achieved. Approximate oligomer yield was 58%. The freeze-dried oligomers precipitates were solubilized in DMSO. The solution concentration was brought to approximately 2 μg/μl. The solution was mixed with 1:1 acetonitrile:water mixture containing 0.1% acetic acid. The total fluid volume entering the ESI-MS was maintained at 0.2 mL min.−1 The positive mass spectrum of the leucine oligomers is shown in FIG. 33A.

后处理

  1. customThe overall synthesis and purification approach
  2. customReaction rates similar to

来源与证据

ORD记录
ord-2f5b427d7bed48daa1186d8c9b6dfb35
来源数据集
uspto-grants-2003_08
专利号
US06605590B1

关联笔记

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