L-Lysine → D-赖氨酸盐酸盐

Into a shaking flask having a volume of 500 ml was charged ml of a medium (pH 7.0) comprising 0.5% of DL-lysine monohydrochloride, 1.0% of polypeptone, 1.0% of a yeast extract and 0.5% of sodium chloride, and the medium was sterilized at 120° C. for 10 minutes. A loopful of Pseudomonas sp. ATCC 14676 was inoculated into the medium and cultured at 30° C. for 20 hours. The cell collected from 1000 ml of the above culture broth by centrifugation was suspended in a physiological saline and then collected by centrifugation. To the cell was added 500 ml of a 50 mM phosphate buffer (pH 7.0) containing 50 g of DL-lysine monohydrochloride, and the mixture was reacted at 30° C. for 72 hours to degrade L-lysine completely. After the reaction, the cells were removed by centrifugation, and subsequent procedures were carried out in the same manner as in Example 1 to obtain 14.2 g of D-lysine monohydrochloride.

1. customInto a shaking flask having
2. additiona volume of 500 ml was charged ml of a medium (pH 7.0)
3. extractionextract
4. waitcultured at 30° C. for 20 hours
5. customThe cell collected from 1000 ml of the above culture broth by centrifugation
6. customcollected by centrifugation
7. additionTo the cell was added 500 ml of a 50 mM phosphate buffer (pH 7.0)
8. additioncontaining 50 g of DL-lysine monohydrochloride
9. customthe mixture was reacted at 30° C. for 72 hours
10. customAfter the reaction
11. customthe cells were removed by centrifugation, and subsequent procedures

ORD记录

ord-bea45d3f17ff4aeeb2836945fd80d389

来源数据集

uspto-grants-1998_03

DOI

10.6084/m9.figshare.5104873.v1

专利号

US05723321