2-Brom-4,6-dinitroanilin

less than 1 mg/mL at 70 °F (NTP, 1992)

SOL IN HOT ACETIC ACID; VERY SOL IN HOT ALCOHOL & ACETONE

307 to 309 °F (NTP, 1992)

153 °C

2-bromo-4,6-dinitroaniline is a bright yellow powder. (NTP, 1992)

Yellow solid; [HSDB] Powder; [MSDSonline]

Danger

H228 (16.7%): Flammable solid [Danger Flammable solids]

P203, P210, P240, P241, P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P318, P319, P321, P332+P317, P337+P317, P362+P364, P370+P378, P403+P233, P405, and P501 (click each P-code to see the statement)

Aggregated GHS information provided per 6 reports by companies from 3 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.

Status: Active Update: 29-07-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/33891

Benzenamine, 2-bromo-4,6-dinitro-: Does not have an individual approval but may be used under an appropriate group standard

A halogenated and nitrated aryl amine. Amines are chemical bases. They neutralize acids to form salts plus water. These acid-base reactions are exothermic. The amount of heat that is evolved per mole of amine in a neutralization is largely independent of the strength of the amine as a base. Amines may be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen is generated by amines in combination with strong reducing agents, such as hydrides.

EYES: First check the victim for contact lenses and remove if present. Flush victim's eyes with water or normal saline solution for 20 to 30 minutes while simultaneously calling a hospital or poison control center. Do not put any ointments, oils, or medication in the victim's eyes without specific instructions from a physician. IMMEDIATELY transport the victim after flushing eyes to a hospital even if no symptoms (such as redness or irritation) develop.

SMALL SPILLS AND LEAKAGE: If a spill of this chemical occurs, FIRST REMOVE ALL SOURCES OF IGNITION, then you should dampen the solid spill material with acetone and transfer the dampened material to a suitable container. Use absorbent paper dampened with acetone to pick up any remaining material. Seal your contaminated clothing and the absorbent paper in a vapor-tight plastic bag for eventual disposal. Solvent wash all contaminated surfaces with acetone followed by washing with a soap and water solution. Do not reenter the contaminated area until the Safety Officer (or other responsible person) has verified that the area has been properly cleaned.

RECOMMENDED RESPIRATOR: Where the neat test chemical is weighed and diluted, wear a NIOSH-approved half face respirator equipped with an organic vapor/acid gas cartridge (specific for organic vapors, HCl, acid gas and SO2) with a dust/mist filter. (NTP, 1992)

The ability of 2-bromo-4,6-dinitroaniline (BDNA) to induce morphological transformation in the BALB/3T3 mouse cell line (Cell Transformation Assay) was evaluated in the absence of added metabolic activation. Based on preliminary clonal toxicity determinations (exposure time=24 hrs), BDNA, in 0.1% (v/v) aqueous acetone, was tested at 5, 15 and 50 ug/ml, with cell survival ranging from 89.6-36.9% relative to solvent control. None of the tested concentrations produced significantly greater transformation frequencies (p > 0.05, Modified Poisson Distribution) relative to the solvent control.

The ability of 2-bromo-4,6-dinitroaniline (BDNA) to induce morphological transformation in the BALB/3T3 mouse cell line (Cell Transformation Assay) was evaluated in the absence of added metabolic activation. Based on preliminary clonal toxicity determinations (exposure time=24 hrs), BDNA, in 0.1% (v/v) aqueous acetone, was tested at 5, 15 and 50 ug/ml, with cell survival ranging from 94.0-77.3% relative to solvent control. At 15 ug/ml, a statistically significant (P<0.05, Modified Poisson Distribution) increase in the number of transformed foci was observed relative to the solvent control.

The ability of 2-bromo-4,6-dinitroaniline (BDNA) to induce morphological transformation in the BALB/3T3 mouse cell line (Cell Transformation Assay) was evaluated in the presence of added metabolic activation by Aroclor-induced rat liver S9 fraction. Based on preliminary clonal toxicity determinations (exposure time=2 hrs), BDNA, in 0.06% (v/v) aqueous acetone, was tested at 3, 10 and 30 ug/ml, with cell survival ranging from 94.8-94.5% relative to solvent control. None of the tested concentrations produced detectable transformation frequencies.

The mutagenicity of 2-bromo-4,6-dinitroaniline (BDNA) was evaluated in Salmonella tester strains TA98, TA100, TA1535, TA1537 and TA1538 (Ames Test), both in the presence and absence of added metabolic activation by Aroclor-induced rat liver S9 fraction. BDNA was tested for mutagenicity at concentrations ranging from 10-1000 ug/plate using the plate incorporation method. BDNA caused a positive response in all of the bacterial tester strains except TA1535, both in the presence and absence of metabolic activation.

The ability of trichlorobutylene oxide (TCBO) to cause unscheduled DNA synthesis in human lung fibroblast cells (WI-38) in vitro was evaluated both with and without metabolic activation by mouse liver S9 fraction. TCBO, dissolved in DMSO, was tested at concentrations of 0, 51, 128, 320, 800 or 2000 ug/ml in the absence of activation and at concentrations of 0, 1447, 1736, 2083, 2500 or 3000 ug/ml with activation. None of the tested concentrations caused an increase in unscheduled DNA synthesis as measured by the amount of tritiated thymidine incorporated into DNA of treated vs control cultures. Evidence of cytotoxicity was observed at each tested concentration.

Used in the synthesis of monoazo dyes; [ChemIDplus] Used as a chemical intermediate for azo derivatives; [HSDB]

CHEM INT FOR AZO DERIVS (PROBABLE AZO DYE INT)

CHEM INT FOR DISPERSE VIOLET 7 & 24-NO CURRENT U.S. PRODN

(1979) No Data

(1981) No Data

(1979) 1.25X10+7 GRAMS (PRINCPL CUSTMS DISTS)

(1981) 4.54X10+7 GRAMS (PRINCPL CUSTMS DISTS)

(1979) PROBABLY GREATER THAN 4.54X10+6 GRAMS

(1981) PROBABLY GREATER THAN 4.54X10+6 GRAMS