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Peptidase, Entero-
RECOMBINANT ENTEROKINASE · EC 3.4.21.9 · ENTEROPEPTIDASE · REK · ENTK · PRSS7 · TMPRSS15 · ENTEROKISE
Identifikatoren & physikalische Daten
| CAS No. | 9014-74-8 |
|---|---|
| Summenformel | C148H230F2O26S8 |
| Durchschnittliche Molmasse | 2.719,9 g/mol |
| Monoisotopische Masse | 2.717,4409131 g/mol |
| SMILES | CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccc(F)cc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccc(F)cc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccccc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccccc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)c1ccccc1.CC(C)CC(C=Cc1ccc(C(C)(C)C)cc1)CCCCS(=O)(=O)O.COc1ccc(CC(C=CC(C)(C)C)CCCCS(=O)(=O)O)cc1.COc1ccc(CC(C=CC(C)(C)C)CCCCS(=O)(=O)O)cc1 |
| InChIKey | VRASXGTWKFQRNS-UHFFFAOYSA-N |
| PubChem CID | 168009870 |
| EC No. | 232-761-1 |
| Lokaler Name | Peptidase, Entero- |
Chemische & physikalische Eigenschaften
| Polarare Oberfläche (TPSA) | 520 |
|---|---|
| H-Brücken-Donoren | 8 |
| H-Brücken-Akzeptoren | 28 |
| Frei drehbare Bindungen | 74 |
| Schwere Atome | 184 |
| Formalladung | 0 |
| Komplexität | 2.230 |
| Lagerbedingungen | -20°C |
| Aggregatzustand | salt-free, lyophilized powder |
| Farbe | white |
| Biologische Quelle | bovine intestine |
| Specific Activity | ≥20units/mg protein |
| CAS Datenbank | 9014-74-8 |
| EPA chemische Informationen | Peptidase, entero- (9014-74-8) |
Sicherheit & Vorschriften
| GHS-Einstufung | DangerH315 (100%): Causes skin irritation [Warning Skin corrosion/irritation]P233, P260, P261, P264, P264+P265, P271, P280, P284, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P342+P316, P362+P364, P403, P403+P233, P405, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 40 reports by companies from 3 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. |
|---|---|
| Regulatorische Informationen | XU - indicates a substance exempt from reporting under the Chemical Data Reporting Rule, (40 CFR 711). |
| Hazard Classes and Categories | Skin Irrit. 2 (100%) |
| Wassergefährdungsklasse (WGK) | 3 |
| TSCA-Inventar-Status | TSCA listed |
| Lagerklasse | 11 - Combustible Solids |
Anwendungen & Verwendung
Verwenden
Typical conditions for fusion protein cleavage:Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl2, and 1% Tween 20 Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mixIncubate reaction mixture at ~25 °C for 16 hours