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Lyase, Chondroitin ABC
EC 4.2.2.4 · units/mg solid · CHONDROITINASE ABC · chondroitinase ABC I · CHONDROITIN ABC LYASE · Lyase, chondroitin ABC · Endolyase, chondroitiBC · chondroitinase-abc bsa free
Identifikatoren & physikalische Daten
| CAS No. | 9024-13-9 |
|---|---|
| Summenformel | C99H144N36 |
| Durchschnittliche Molmasse | 1.838,4 g/mol |
| Monoisotopische Masse | 1.837,23746875 g/mol |
| SMILES | CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1 |
| InChIKey | QSCUSRCQBGTCDB-UHFFFAOYSA-N |
| PubChem CID | 168009893 |
| Lokaler Name | Lyase, Chondroitin ABC |
Chemische & physikalische Eigenschaften
| Polarare Oberfläche (TPSA) | 691 |
|---|---|
| H-Brücken-Donoren | 18 |
| H-Brücken-Akzeptoren | 9 |
| Frei drehbare Bindungen | 36 |
| Schwere Atome | 135 |
| Formalladung | 0 |
| Komplexität | 239 |
| Lagerbedingungen | -20°C |
| Löslichkeit | 0.01% bovine serum albumin aqueous (BSA) solution: soluble |
| Aggregatzustand | lyophilized powder |
| Farbe | White to off-white |
| Biologische Quelle | bacterial (Proteus vulgaris) |
| Specific Activity | 50-250units/mg protein |
| Hauptanwendungen | diagnostic assay manufacturing |
Sicherheit & Vorschriften
| F | 10-21 |
|---|---|
| Wassergefährdungsklasse (WGK) | 3 |
| Sicherheitshinweise (S) | 22-24/25 |
| Lagerklasse | 11 - Combustible Solids |
Anwendungen & Verwendung
Verwenden
Chondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG). The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan.