Albumine, Butserum
Bovine serum albumin · SERUM ALBUMIN · AlbuMin Bovine V · compound amino acid · ALBUMIN FROM BOVINE SERUM · BSAV · Almond protein powder · ALBUMIN, BOVINE (BSA), CRYSTALLINE
Identifikatoren & physikalische Daten
| CAS No. | 9048-46-8 |
|---|---|
| Summenformel | N/A |
| Lokaler Name | Albumine, Butserum |
Chemische & physikalische Eigenschaften
| Lagerbedingungen | 2-8°C |
|---|---|
| Löslichkeit | PBS: >40 mg/mLSoluble in water. |
| Aggregatzustand | lyophilized powder |
| Farbe | clear clear, colorless |
| pH | 6.5-7.5 (1% in 0.15 M sodium chloride) |
| Biologische Quelle | rabbit |
| Merck | 13,8542 |
| Stabilität | Stable. Incompatible with strong oxidizing agents and strong acids. Refrigerate. |
| Kosmetik-Inhaltsstoffe Funktionen | SOLVENTSURFACTANT - CLEANSINGFILM FORMINGSKIN CONDITIONINGHAIR CONDITIONINGHUMECTANT |
| EPA chemische Informationen | Albumins, blood serum (9048-46-8) |
| Absorption | ≤0.4 at 550nm |
Sicherheit & Vorschriften
| F | 10-21 |
|---|---|
| Wassergefährdungsklasse (WGK) | 3 |
| TSCA-Inventar-Status | TSCA listed |
| Zollcode | 35029000 |
| R-Sätze (R) | 22-52/53-21/22 |
| Toxizität | LD50 intravenous in monkey: > 12500mg/kg |
| Gefahrensymbole | Xn |
| Sicherheitshinweise (S) | 24/25-61-36/37 |
| Lagerklasse | 11 - Combustible Solids |
Anwendungen & Verwendung
Beschreibung
Bovine serum albumin is a protein which has a key role in regulating blood colloidal osmotic pressure. A water-soluble protein composed of 583 amino acids, BSA has a calculated molecular weight of 66,430 Daltons. Six α-helices form three homologous domains of BSA. Depending on pH, BSA undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.This BSA product is specifically evaluated and tested for the following properties:Very low protease content / protease-freeVery low fatty acid content / fatty acid-freeProtease-free BSA is especially useful for applications like:Enzyme assaysProtein-based assaysProtease-sensitive techniques that include:a. Enzyme immunoassay (EIA)b. Nucleic acid hybridizationc. Radioimmunoassay (RIA)
Verwenden
Suitable for cell culture media supplement.Bovine serum albumin (BSA) Fraction V has uses in many applications such as stabilizing proteins in solution, blocking non-specific binding in immuno-assays, and as a protein standard in various protein assays.Stabilizer for proteins and enzymes in solutionBlocking agent for immuno-assays (ie, western blot, IHC, IF)Minimizes non-specific antibody bindingIgG- and protease-free50 g of solid powderBovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody. It is also used as a blocking agent to reduce non-specific antibody binding in immuno-detection procedures such as western blotting, immunofluorescence, and IHC. It is also suitable for use in protease sensitive assays such as RIA and EIA, as well as nucleic acid hybridization. This BSA is IgG- and protease-free.