4',6-Diamidino-2-phenylindole dihydrochloride
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Grundinformationen
- CAS
28718-90-3- Standard-SMILES
Cl.Cl.N=C(N)c1cc(C(=N)N)c2cc(-c3ccccc3)[nH]c2c1- Summenformel
- C16H16ClN5
- Durchschnittliche Molmasse
- 313,79 g/mol
Namen und Bezeichner
- Bevorzugter Name
- 4',6-Diamidino-2-phenylindole dihydrochloride
- Englischer Name
- 4',6-Diamidino-2-phenylindole dihydrochloride
- Summenformel
- C16H16ClN5
- Molmasse
- 313.79
Namen und Synonyme
DAPIDAPI.HClDAPI 2HCLD9542-5MGDAPI StainSigma，DAPI-Cellstain-DapiDAPI(meilunbio)DAPI[10236276001]DAPI Stain Solution
Datenbank-Bezeichner
- CAS
28718-90-3- PubChem CID
- 21918207
Eigenschaften
Physikochemische Eigenschaften
- Schmelzpunkt
- 300 °C · >300°C (dec.)
- storage temp.
- 2 · 2-8°C
- Löslichkeit
- 2 O: · H2O: 10 mg/mL heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.
- Aggregatzustand
- powder
- Farbe
- Clear
- PH
- 4 · 4.0-5.0 (10g/l, H2O, 20℃)
- Wasserlöslichkeit
- water: soluble
- BRN
- 4894417 · 4894417
- Stabilität
- 2 years · PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months.
- InChI
- InChI=1S/C16H15N5.ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;/h1-8,21H,(H3,17,18)(H3,19,20);1H
- InChIKey
- FPNZBYLXNYPRLR-UHFFFAOYSA-N
- SMILES
- C(C1C=CC2C=C(C3C=CC(C(N)=N)=CC=3)NC=2C=1)(N)=N.Cl
- CAS Datenbank
- 28718 · 28718-90-3(CAS DataBase Reference)
Sicherheit und Regulatorisches
Sicherheitsangaben dienen nur zur Orientierung und ersetzen weder das Sicherheitsdatenblatt (SDB) des Lieferanten noch geltende Vorschriften oder eine fachliche Bewertung.
Sicherheitsdaten
- F
- 3-8-10-21
- wgk
- 3
- hs_code
- 29339900
- RTECS-Nr.
- NL5995050
- risk_code
- 36/37/38-20/21/22
- hazard_code
- Xi,Xn
- safety_code
- 26-36-24/25-22-37/39
- Speicherklasse
- 11 - Combustible Solids
- Hazard Classifications
- Skin Irrit. 2Skin Sens. 1ASTOT SE 3
Chemie und Industrie
Anwendungen
BeschreibungDAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences of DNA. DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.
Verwenden4',6-Diamidino-2-phenylindole dihydrochloride is used as a cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine.It is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.
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