Sodium pyruvate

BeschreibungSodium pyruvate is the sodium salt of pyruvate. It is frequently supplemented to the cell culture medium to act as a source of energy since it is a key intermediate during the production of the high-energy ATP molecules inside cells. For example, it can be used as a carbon source for bacteria. It may also protect cell against hydrogen peroxide, an oxidant to scavenger oxygen radicals. It is an important metabolic intermediate in many essential metabolic pathways such as carbohydrate metabolism. For example, it is converted into acetyl coenzyme A and enters into the TCA cycle (Kreb’s cycle) in organisms. It is also involved in the amino acid metabolism in organisms.

VerwendenSodium pyruvate is used in cell culture media as an additional source of energy. It acts as an antioxidant and finds protective effects against oxygen radicals. It serves as an intermediate in many metabolic pathways such as sugar metabolism. It is converted into acetyl coenzyme A and enters the trichloroacetic acid cycle. In addition, it is involved with amino acid metabolism and initiates the Kreb's cycle. It plays an important role as a free radical scavenger.

synthetischeThe preparation of sodium pyruvate is as follows:Synthesis of pyruvate, a salt of a compound of formula (II) by ozonolysis of methacrylic acid, a compound of formula (II) A solution of methacrylic acid obtained in Example la or lb (15.31 g, 178 mmol) in dichloromethane/methanol (5 % methanol, 50 ml) was cooled to -78 0C and a stream of ozone was passed through until the solution turned blue. Dimethylsulfide (12.41 g, 200 mmol) was added and the mixture was allowed to reach room temperature. Excess dimethylsulfide was removed by passing a stream of nitrogen through the reaction mixture. The reaction mixture was evaporated in vacuo at 30 0C. To the residue was added water (100 ml) and then slowly added a solution of aq. NaOH (IM, 178 ml). The mixture was concentrated and left for crystallisation at 4 °C. The white crystalline material obtained was filtered and washed with acetone (3x100 ml). Yield: 14.50 g (92 %). Purity: 95 %.