4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure
HEPES BUFFER · 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID · HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID · 4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID · hepes solution · Biological Buffer · HEPES buffer Solution · EPES
Identifikatoren & physikalische Daten
| CAS No. | 7365-45-9 |
|---|---|
| Summenformel | C8H18N2O4S |
| Durchschnittliche Molmasse | 238,31 g/mol |
| Monoisotopische Masse | 238,09872823 g/mol |
| SMILES | O=S(=O)([O-])CCN1CC[NH+](CCO)CC1 |
| InChIKey | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| PubChem CID | 23830 |
| ChEBI | CHEBI:46755 |
| Lokaler Name | 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure |
Chemische & physikalische Eigenschaften
| XLogP | -4,1 |
|---|---|
| Polarare Oberfläche (TPSA) | 93,5 |
| H-Brücken-Donoren | 2 |
| H-Brücken-Akzeptoren | 5 |
| Frei drehbare Bindungen | 4 |
| Schwere Atome | 15 |
| Formalladung | 0 |
| Komplexität | 254 |
| Schmelzpunkt | 234-238 °C |
| Siedepunkt | 408℃[at 101 325 Pa] |
| Dichte | 1.07 g/mL at 20 °C |
| Dampfdruck | 0Pa at 25℃ |
| Brechungsindex | n20/D 1.339 |
| Lagerbedingungen | 2-8°C |
| Löslichkeit | H2O: 1 M at 20 °C, clear, colorlessSoluble |
| Aggregatzustand | powder |
| Farbe | White |
| pH | 5.0-6.5 (1Maqueous solution) |
| pKa (Säurekonstante) | 7.5(at 25℃) |
| Biologische Quelle | synthetic |
| Merck | 14,4654 |
| BRN | 883043 |
| Stabilität | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
| Hauptanwendungen | diagnostic assay manufacturing |
| Kosmetik-Inhaltsstoffe Funktionen | BUFFERING |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) |
| InChIKey | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| SMILES | OCCN1CCN(CC1)CCS(O)(=O)=O |
| LogP (Verteilungskoeffizient) | -3.85 at 20℃ |
| CAS Datenbank | 7365-45-9(CAS DataBase Reference) |
| EPA chemische Informationen | 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9) |
| Absorption | ≤0.05 at 290 in H2O at 33% |
Sicherheit & Vorschriften
| Wassergefährdungsklasse (WGK) | 1 |
|---|---|
| TSCA-Inventar-Status | TSCA listed |
| Zollcode | 29335995 |
| RTECS-Nr. | TL6809000 |
| R-Sätze (R) | 36/37/38 |
| Gefahrensymbole | Xi |
| Sicherheitshinweise (S) | 24/25-22-36-26 |
| Lagerklasse | 13 - Non Combustible Solids |
Anwendungen & Verwendung
Beschreibung
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Verwenden
Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.