Hydrogen[4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulfonatobenzyl)amino]-2'-methylbenzhydrylen]-3-methylcyclohexa-2,5-dien-1-yliden](ethyl)(m-sulfonatobenzyl)ammonium, Mononatriumsalz

Acid Blue 90

HCID 6333920

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Grundinformationen

CAS
6104-58-1
Standard-SMILES
CCOc1ccc(Nc2ccc(/C(=C3\C=CC(=[N+](CC)Cc4cccc(S(=O)(=O)O)c4)C=C3C)c3ccc(N(CC)Cc4cccc(S(=O)(=O)O)c4)cc3C)cc2)cc1
InChIKey
JFNOCRSXNFZMNT-UHFFFAOYSA-M
Summenformel
C47H48N3NaO7S2
Durchschnittliche Molmasse
854,02 g/mol

Namen und Bezeichner

Bevorzugter Name
Acid Blue 90
Lokaler Name
Hydrogen[4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulfonatobenzyl)amino]-2'-methylbenzhydrylen]-3-methylcyclohexa-2,5-dien-1-yliden](ethyl)(m-sulfonatobenzyl)ammonium, Mononatriumsalz
Englischer Name
Acid Blue 90
Summenformel
C47H48N3NaO7S2
Molmasse
854.02

Namen und Synonyme

BRILLIANT BLUEBRILLIANT BLUE GCOOMASSIE BRILLIANT BLUE G-250G-250COOMASSIE BRILLIANT BLUE GCI 42655CBB G-250Cyanine GBlue G 250CI NO 42655

Datenbank-Bezeichner

CAS
6104-58-1
PubChem CID
6333920

Eigenschaften

Physikochemische Eigenschaften

Schmelzpunkt
100 °C · 100 °C
Dichte
1.274 · 1.274[at 20℃]
Dampfdruck
0.093 Pa · 0.093Pa at 20℃
Flammpunkt
11 °C · 11 °C
storage temp.
2 · 2-8°C
Löslichkeit
2 O: · H2O: soluble1mg/mL

Sicherheit und Regulatorisches

Sicherheitsdaten

wgk
3
tsca
TSCA listed
hs_code
32041200
Speicherklasse
11 - Combustible Solids

Chemie und Industrie

Anwendungen · 1 Eintrag

VerwendenBrilliant Blue G is used in analytical biochemistry dye and as a P2X7 antagonist. It is also used in protein staining following gel electrophoresis. It is used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery. It is useful for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualized during staining. It has been used in the Bradford dye-binding protein assay. A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents.

Darstellung · 1 Eintrag

synthetischeBenzaldehyde (1 Moore) and N-ethyl-N-benzyl-3-methylaniline(2 Moore) condensation, And then sulfonating the product of three acids, and lead oxide, peroxide, and then the reaction with the 4-Ethoxybenzenamine, Benzaldehyde sulfonic acid groups on the phenyl ring is substituted anilino-ethoxy.

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