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BeschreibungAlcohol oxidase (AOX, EC 1.1.3.13) is a flavoenzyme, an oxidoreductase that catalyzes the oxidation of alcohol to the corresponding carbonyl compound and simultaneously releases hydrogen peroxide.Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to the respective aldehydes.RCH2OH + O2 -->RCHO + H2O2The enzyme has the highest affinity for methanol with the affinity decreasing with increasing chain length of the alkyl (R) group.Alcohol oxidase plays a major role in the metabolism of methanol resulting in the formation of formaldehyde and has been detected in several genera of yeasts, such as Candida, Pichia, and Hansenula, that utilize methanol as a sole carbon and energy source.Primarily localized in the peroxisome, alcohol oxidase has also been found in the cytoplasm. Monomers are synthesized in the cytosol and assembled into octomers in the peroxisome. Octomerization is thought to be chaparone mediated. Alcohol oxidase is of interest for the study of protein translocation into peroxisomes.
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