4-Morpholineethanesulfonic acid
MES HYDRATE · MES.H2O · MES 1HYD FREE ACID XTL · MORPHOLINOETHANE SULFONIC ACID MONOHYDRATE · 2-(N-MORPHOLINO)ETHANESULFONIC ACID HYDRATE · 4-MORPHOLINEETHANESULFONIC ACID MONOHYDRATE · MES-H · MES-HO
Identifikatoren & physikalische Daten
| CAS No. | 145224-94-8 |
|---|---|
| Summenformel | C6H13NO4S |
| Durchschnittliche Molmasse | 195,24 g/mol |
| Monoisotopische Masse | 195,0565289 g/mol |
| SMILES | O=S(=O)(O)CCN1CCOCC1 |
| InChIKey | SXGZJKUKBWWHRA-UHFFFAOYSA-N |
| PubChem CID | 78165 |
| ChEMBL | CHEMBL1234276 |
| ChEBI | CHEBI:39005 |
| EC No. | 224-632-3 |
| DTXSID | DTXSID4063454 |
Chemische & physikalische Eigenschaften
| XLogP | -3,5 |
|---|---|
| Polarare Oberfläche (TPSA) | 75,2 |
| H-Brücken-Donoren | 1 |
| H-Brücken-Akzeptoren | 5 |
| Frei drehbare Bindungen | 3 |
| Schwere Atome | 12 |
| Formalladung | 0 |
| Komplexität | 214 |
| Stoffklassen | Other Uses -> Biological Buffers |
| Arzneimittel | Pharmaceuticals -> Listed in ZINC15 |
| Physical Description | Colorless solid[Merck Index] White powder[Aldrich MSDS] |
| Schmelzpunkt | >300 °C(lit.) |
| Dichte | 10.66g/mL |
| Lagerbedingungen | Sealed in dry,Room Temperature |
| Löslichkeit | H2O: 0.5 M at 20 °C, clearSoluble in water. |
| Aggregatzustand | Powder, Crystals or Flakes |
| Farbe | White to pale yellow and/or orange |
| Geruch | Odorless |
| pH | 2.5-4.0 (25℃, 0.5M in H2O) |
| pKa (Säurekonstante) | 6.1(at 25℃) |
| Biologische Quelle | synthetic |
| Merck | 14,5902 |
| BRN | 141319 |
| Hauptanwendungen | agriculturediagnostic assay manufacturinggeneral analyticalmicrobiology |
| InChI | InChI=1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10)1H2 |
| InChIKey | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| SMILES | S(=O)(=O)(O)CCN1CCOCC1.O |
| CAS Datenbank | 145224-94-8(CAS DataBase Reference) |
Sicherheit & Vorschriften
| GHS-Einstufung | This chemical does not meet GHS hazard criteria for 97.3% (252 of 259) of all reports.Not ClassifiedAggregated GHS information provided per 259 reports by companies from 7 notifications to the ECHA C&L Inventory.This chemical does not meet GHS hazard criteria for 2.1% (2 of 96) of reports.WarningH315 (97.9%): Causes skin irritation [Warning Skin corrosion/irritation] |
|---|---|
| Regulatorische Informationen | Chemical: 4-Morpholineethanesulfonic acidStatus: Active Update: 01-12-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/247144-Morpholineethanesulfonic acid: Does not have an individual approval but may be used under an appropriate group standard |
| Gefahrenübersicht | May cause irritation[Aldrich MSDS] See Morpholine. |
| Hazard Classes and Categories | Not ClassifiedSkin Irrit. 2 (100%)Skin Irrit. 2 (97.9%) |
| Wassergefährdungsklasse (WGK) | 3 |
| TSCA-Inventar-Status | Yes |
| Zollcode | 29349990 |
| RTECS-Nr. | QE3479500 |
| R-Sätze (R) | 36/37/38 |
| Gefahrensymbole | Xi |
| Sicherheitshinweise (S) | 26-36-37/39 |
| Lagerklasse | 11 - Combustible Solids |
Anwendungen & Verwendung
Beschreibung
MES Monohydrate is a zwitterionic buffer that is not absorbed through cell membranes and is virtually transparent in UV light. MES is a buffering agent used in many biological and biochemical applications. It is also used as a running buffer for denaturing gel electrophoresis. The characteristics of low UV absorptivity, minimal reactivity, stable pH and solubility in water allow MES Monohydrate to be used as a Good's buffer.
Verwenden
MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.