2,2'-(Piperazin-1,4-diyl)bis(ethansulfonsaeure)
PIPES FREE ACID · 1,4-Piperazinebis(ethanesulphonic acid) · PIPES · PIPES,98% · PIPES0215 · PIPES, 99% · Pipes AR 98% · PIPES【Dotite】
Identifikatoren & physikalische Daten
| CAS No. | 5625-37-6 |
|---|---|
| Summenformel | C8H18N2O6S2 |
| Durchschnittliche Molmasse | 302,4 g/mol |
| Monoisotopische Masse | 302,0606283 g/mol |
| SMILES | O=S(=O)(O)CCN1CCN(CCS(=O)(=O)O)CC1 |
| InChIKey | IHPYMWDTONKSCO-UHFFFAOYSA-N |
| PubChem CID | 79723 |
| ChEBI | CHEBI:44933 |
| EC No. | 227-057-6 |
| DTXSID | DTXSID8063965 |
| Lokaler Name | 2,2'-(Piperazin-1,4-diyl)bis(ethansulfonsaeure) |
Chemische & physikalische Eigenschaften
| XLogP | -6,7 |
|---|---|
| Polarare Oberfläche (TPSA) | 132 |
| H-Brücken-Donoren | 2 |
| H-Brücken-Akzeptoren | 8 |
| Frei drehbare Bindungen | 6 |
| Schwere Atome | 18 |
| Formalladung | 0 |
| Komplexität | 397 |
| Stoffklassen | Other Uses -> Biological Buffers |
| Physical Description | White powder[Alfa Aesar MSDS] |
| Schmelzpunkt | >300 °C (lit.) |
| Dichte | 1.4983 (rough estimate) |
| Brechungsindex | 1.6300 (estimate) |
| Lagerbedingungen | Sealed in dry,Room Temperature |
| Löslichkeit | 0.1 M NaOH: 0.25 g/mL, clear, colorlessSoluble in water. |
| Aggregatzustand | Micro-Crystalline Powder |
| Farbe | White |
| pKa (Säurekonstante) | 6.8(at 25℃) |
| pH | 6.1-7.5 |
| Merck | 14,7479 |
| BRN | 817713 |
| Hauptanwendungen | diagnostic assay manufacturing |
| InChI | 1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16) |
| InChIKey | XWGRYGKFRRLUQQ-UHFFFAOYSA-N |
| SMILES | OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O |
| LogP (Verteilungskoeffizient) | -2.7 at 20℃ |
| CAS Datenbank | 5625-37-6(CAS DataBase Reference) |
| EPA chemische Informationen | 1,4-Piperazinediethanesulfonic acid (5625-37-6) |
| Absorption | ≤0.1 at 260 in 1 M NaOH at 0.5M≤0.1 at 280 in 1 M NaOH at 0.5M |
Sicherheit & Vorschriften
| GHS-Einstufung | This chemical does not meet GHS hazard criteria for 96% (72 of 75) of all reports.Not ClassifiedAggregated GHS information provided per 75 reports by companies from 3 notifications to the ECHA C&L Inventory. |
|---|---|
| Regulatorische Informationen | Chemical: 1,4-Piperazinediethanesulfonic acidStatus: Active Update: 25-08-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/224441,4-Piperazinediethanesulfonic acid: Does not have an individual approval but may be used under an appropriate group standard |
| Gefahrenübersicht | May cause irritation[Alfa Aesar MSDS] |
| Hazard Classes and Categories | Not Classified |
| Wassergefährdungsklasse (WGK) | 3 |
| TSCA-Inventar-Status | TSCA listed |
| Zollcode | 29335995 |
| R-Sätze (R) | 36/37/38 |
| Gefahrensymbole | Xi |
| Sicherheitshinweise (S) | 22-24/25-37/39-26 |
| Lagerklasse | 11 - Combustible Solids |
Anwendungen & Verwendung
Beschreibung
PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.
Verwenden
A buffering agent with a pKa near physiological pH.PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.Additional forms available: PIPES, Sesquisodium Salt ; PIPES dipotassium salt; PIPES disodium salt; PIPES, Sodium Salt. Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.