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N-(Tri(hydroxymethyl)methyl)glycin
TRIS-HCL BUFFER · 1389475 · TROMETHAMINE HYDROCHLORIDE · Ticine · Tricine buffer, free acid · N-[TRIS(HYDROXYMETHYL)METHYL]GLYCINE · TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE · TRICINE
Identifikatoren & physikalische Daten
| CAS No. | 5704-04-1 |
|---|---|
| Summenformel | C6H13NO5 |
| Durchschnittliche Molmasse | 179,17 g/mol |
| Monoisotopische Masse | 179,07937252 g/mol |
| SMILES | O=C(O)CNC(CO)(CO)CO |
| InChIKey | SEQKRHFRPICQDD-UHFFFAOYSA-N |
| PubChem CID | 79784 |
| ChEBI | CHEBI:39063 |
| EC No. | 227-193-6 |
| DTXSID | DTXSID1063982 |
| Lokaler Name | N-(Tri(hydroxymethyl)methyl)glycin |
Chemische & physikalische Eigenschaften
| XLogP | -5,1 |
|---|---|
| Polarare Oberfläche (TPSA) | 110 |
| H-Brücken-Donoren | 5 |
| H-Brücken-Akzeptoren | 6 |
| Frei drehbare Bindungen | 6 |
| Schwere Atome | 12 |
| Formalladung | 0 |
| Komplexität | 137 |
| Physical Description | Solid[Merck Index] White crystals[Sigma-Aldrich MSDS] |
| Schmelzpunkt | 186-188 °C (dec.) |
| Siedepunkt | 311.69°C (rough estimate) |
| Dichte | 1.05 g/mL at 20 °C |
| Dampfdruck | 0.002-0.004Pa at 20-25℃ |
| Brechungsindex | 1.4240 (estimate) |
| Lagerbedingungen | Keep in dark place,Inert atmosphere,Room temperature |
| Löslichkeit | H2O: 4 M at 20 °C, clear, colorlesssoluble |
| Aggregatzustand | crystalline |
| Farbe | clear colorless (40 % (w/w) in H2O) solution |
| Geruch | Odorless |
| pH | 4.0-6.0 (1M in H2O) |
| pKa (Säurekonstante) | 8.1(at 25℃) |
| Biologische Quelle | synthetic |
| Merck | 14,9651 |
| BRN | 1937804 |
| Stabilität | Stable. |
| InChI | 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12) |
| InChIKey | SEQKRHFRPICQDD-UHFFFAOYSA-N |
| SMILES | OCC(CO)(CO)NCC(O)=O |
| LogP (Verteilungskoeffizient) | -2.87 at 25℃ |
| CAS Datenbank | 5704-04-1(CAS DataBase Reference) |
| EPA chemische Informationen | Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1) |
| Absorption | ≤0.05 at 280 in H2O at 1M≤0.1 at 260 in H2O at 1M |
Sicherheit & Vorschriften
| GHS-Einstufung | This chemical does not meet GHS hazard criteria for 97.6% (121 of 124) of all reports.Not ClassifiedReported as not meeting GHS hazard criteria by 121 of 124 companies (only 2.4% companies provided GHS information). For more detailed information, please visit ECHA C&L website.Aggregated GHS information provided per 124 reports by companies from 4 notifications to the ECHA C&L Inventory.Reported as not meeting GHS hazard criteria per 121 of 124 reports by companies.There are 3 notifications provided by 3 of 124 reports by companies with hazard statement code(s).Information may vary between notifications depending on impurities, additives, and other factors. The percentage value in parenthesis indicates the notified classification ratio from companies that provide hazard codes. Only hazard codes with percentage values above 10% are shown. For more detailed information, please visit ECHA C&L website. |
|---|---|
| Regulatorische Informationen | Chemical: Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-Status: Active Update: 02-03-2021 https://echa.europa.eu/registration-dossier/-/registered-dossier/25731Glycine, N-2-hydroxy-1,1-bis(hydroxymethyl)ethyl-: Does not have an individual approval but may be used under an appropriate group standard |
| Weitere Sicherheitsinformationen | Evaluation - Chemicals that are unlikely to require further regulation to manage risks to environment |
| Gefahrenübersicht | May cause irritation[Sigma-Aldrich MSDS] |
| Hazard Classes and Categories | Not Classified |
| F | 3 |
| Wassergefährdungsklasse (WGK) | 3 |
| TSCA-Inventar-Status | TSCA listed |
| Zollcode | 29052900 |
| R-Sätze (R) | 36/37/38 |
| Gefahrensymbole | Xi |
| Sicherheitshinweise (S) | 26-36-36/37/39 |
| Lagerklasse | 13 - Non Combustible Solids |
Anwendungen & Verwendung
Verwenden
Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).