N,N-Bis(2-hydroxyethyl)glycin
BICINE BUFFER · N,N-DI(2-HYDROXYETHYL)GLYCINE · N,N-BIS(2-HYDROXYETHYL)GLYCINE · BIClNE · BICINE · Bicirne · Biobuffer · BICINE, 99+%
Identifikatoren & physikalische Daten
| CAS No. | 150-25-4 |
|---|---|
| Summenformel | C6H13NO4 |
| Durchschnittliche Molmasse | 163,17 g/mol |
| Monoisotopische Masse | 163,0844579 g/mol |
| SMILES | O=C(O)CN(CCO)CCO |
| InChIKey | FSVCELGFZIQNCK-UHFFFAOYSA-N |
| PubChem CID | 8761 |
| ChEMBL | CHEMBL1231251 |
| ChEBI | CHEBI:40957 |
| EC No. | 205-755-1 |
| DTXSID | DTXSID3041669 |
| Lokaler Name | N,N-Bis(2-hydroxyethyl)glycin |
Chemische & physikalische Eigenschaften
| XLogP | -3,8 |
|---|---|
| Polarare Oberfläche (TPSA) | 81 |
| H-Brücken-Donoren | 3 |
| H-Brücken-Akzeptoren | 5 |
| Frei drehbare Bindungen | 6 |
| Schwere Atome | 11 |
| Formalladung | 0 |
| Komplexität | 111 |
| Stoffklassen | Other Uses -> Biological Buffers |
| Arzneimittel | Pharmaceuticals -> Listed in ZINC15 |
| Physical Description | Solid[Merck Index] White crystalline powder[Alfa Aesar MSDS] |
| Schmelzpunkt | 190 °C (dec.)(lit.) |
| Siedepunkt | 290.25°C (rough estimate) |
| Dichte | 1.05 g/mL at 20 °C |
| Dampfdruck | 0.002-0.007Pa at 20-25℃ |
| Brechungsindex | 1.4240 (estimate) |
| Lagerbedingungen | room temp |
| Löslichkeit | H2O: 1 M at 20 °C, clear, colorlesssoluble |
| Aggregatzustand | Powder |
| Farbe | White |
| pH | 4.0-5.5 (25℃, 1M in H2O) |
| pKa (Säurekonstante) | 8.3(at 25℃) |
| Merck | 14,1201 |
| BRN | 1769362 |
| InChI | 1S/C6H13NO4/c8-3-1-7(2-4-9)5-6(10)11/h8-9H,1-5H2,(H,10,11) |
| InChIKey | FSVCELGFZIQNCK-UHFFFAOYSA-N |
| SMILES | OCCN(CCO)CC(O)=O |
| LogP (Verteilungskoeffizient) | 2.3 at 20℃ and pH7 |
| Oberflächenspannung | 72.2mN/m at 1g/L and 20℃ |
| CAS Datenbank | 150-25-4(CAS DataBase Reference) |
| EPA chemische Informationen | Bicine (150-25-4) |
| Absorption | ≤0.05 at 260 in H2O at 1M≤0.05 at 280 in H2O at 1M |
Sicherheit & Vorschriften
| GHS-Einstufung | This chemical does not meet GHS hazard criteria for 97.6% (124 of 127) of all reports.Not ClassifiedAggregated GHS information provided per 127 reports by companies from 4 notifications to the ECHA C&L Inventory. |
|---|---|
| Regulatorische Informationen | Chemical: Glycine, N,N-bis(2-hydroxyethyl)-Status: Active Update: 23-06-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/23693Glycine, N,N-bis(2-hydroxyethyl)-: Does not have an individual approval but may be used under an appropriate group standard |
| Weitere Sicherheitsinformationen | Evaluation - Chemicals that are unlikely to require further regulation to manage risks to environment |
| Gefahrenübersicht | Causes changes in tubules (including acute renal failure and necrosis) in intraperitoneal lethal-dose studies of mice (LD50 = 1,540 mg/kg)[RTECS] May cause irritation[Alfa Aesar MSDS] See Glycine. |
| Hazard Classes and Categories | Not Classified |
| Unerwünschte Wirkungen | Nephrotoxin - The chemical is potentially toxic to the kidneys in the occupational setting. |
| F | 3-10 |
| Wassergefährdungsklasse (WGK) | 3 |
| TSCA-Inventar-Status | TSCA listed |
| Zollcode | 29225000 |
| RTECS-Nr. | MB9700000 |
| R-Sätze (R) | 20/21/22-36/37/38 |
| Toxizität | mouse,LD50,intraperitoneal,1540mg/kg (1540mg/kg),KIDNEY, URETER, AND BLADDER: "CHANGES IN TUBULES (INCLUDING ACUTE RENAL FAILURE, ACUTE TUBULAR NECROSIS)",Revue d'Epidemiologie, Medecine Sociale et Sante Publique. Vol. 10, Pg. 391, 1962. |
| Gefahrensymbole | Xn |
| Sicherheitshinweise (S) |
Anwendungen & Verwendung
Beschreibung
Bicine is an amino acid that results from amine degradation due to the presence of oxygen and/or sulfur dioxide. The amine solvents that undergo this degradation are MDEA, DEA, TEA, and mixed amine solvents containing any of these amines. As a zwitterionic amino acid buffer agent, bicine is active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Bicine is a recommended buffer for low-temperature biochemical work. It is used for the preparation of stable substrate solution for serum guanase determination. It can be used as a nitrogenous strength enhancer to regulate cement hydration and improve cement properties. Studies have found that bicine significantly changes the morphology of portlandite crystals from a parallel-stacked lamellar shape to a distorted one and also decreases the pore size of the hardened cement paste at an early age[1]. In particular, bicine could promote the rapid formation of β-sheet-rich amyloid-β fibrils[2].
Verwenden
A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.