2-Amino-2-(hydroxymethyl)propan-1,3-diolhydrochlorid
TRIS-HCL · Tris-HCI · TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE · TRIZMA HYDROCHLORIDE · TRIS-HCL BUFFER · Tris-HC · TRIS-HC1 · Tris hydrochloric acid
Identifikatoren & physikalische Daten
| CAS No. | 1185-53-1 |
|---|---|
| Summenformel | C4H11NO3.ClH |
| Durchschnittliche Molmasse | 157,59 g/mol |
| Monoisotopische Masse | 157,05057092 g/mol |
| SMILES | Cl.NC(CO)(CO)CO |
| InChIKey | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| PubChem CID | 93573 |
| ChEMBL | CHEMBL3183206 |
| EC No. | 214-684-5 |
| DTXSID | DTXSID4044373 |
| Lokaler Name | 2-Amino-2-(hydroxymethyl)propan-1,3-diolhydrochlorid |
Chemische & physikalische Eigenschaften
| Polarare Oberfläche (TPSA) | 86,7 |
|---|---|
| H-Brücken-Donoren | 5 |
| H-Brücken-Akzeptoren | 4 |
| Frei drehbare Bindungen | 3 |
| Schwere Atome | 9 |
| Formalladung | 0 |
| Komplexität | 54 |
| Stoffklassen | Nitrogen Compounds -> Amino Alcohols, Other |
| Physical Description | Dry PowderLiquidOther SolidHygroscopic solid[Sigma-Aldrich MSDS] |
| Schmelzpunkt | 150-152 °C |
| Siedepunkt | 225℃[at 101 325 Pa] |
| Dichte | 1.05 g/mL at 20 °C |
| Dampfdruck | 0Pa at 20℃ |
| Lagerbedingungen | room temp |
| Löslichkeit | H2O: 4 M at 20 °C, clear, colorlessSOLUBLE |
| Aggregatzustand | crystalline |
| Farbe | clear colorless (40 % (w/w) in H2O) solution |
| pH | 2.5-4.0 (25℃, 4M in H2O) |
| pKa (Säurekonstante) | 8.1(at 25℃) |
| Biologische Quelle | synthetic |
| BRN | 3675235 |
| Stabilität | Stable. Incompatible with strong oxidizing agents, bases, moisture. |
| Kosmetik-Inhaltsstoffe Funktionen | ANTIMICROBIAL |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H21H |
| InChIKey | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| SMILES | Cl.NC(CO)(CO)CO |
| LogP (Verteilungskoeffizient) | -3.6 at 20℃ |
| CAS Datenbank | 1185-53-1(CAS DataBase Reference) |
| EPA chemische Informationen | 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1185-53-1) |
| Absorption | ≤0.05 at 290nm at 40% |
Sicherheit & Vorschriften
| GHS-Einstufung | This chemical does not meet GHS hazard criteria for 57.2% (103 of 180) of all reports.WarningH315 (42.2%): Causes skin irritation [Warning Skin corrosion/irritation]P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 180 reports by companies from 16 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. |
|---|---|
| Regulatorische Informationen | Chemical: 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1) is listed on the EPA's Chemical Data Reporting (CDR) system. Manufacturers and importers of 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1) are required to report information about their production and use of this chemical to the EPA under the Toxic Substances Control Act (TSCA). (40 eCFR Part 711)Status: Active Update: 09-12-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/193062-amino-2-(hydroxymethyl)propane-1,3-diol: Does not have an individual approval but may be used under an appropriate group standard |
| Gefahrenübersicht | A mild eye irritant in rabbitsNot a sensitizer in experimental animals[Sigma-Aldrich MSDS] See Tromethamine. |
| Hazard Classes and Categories | Skin Irrit. 2 (42.2%) |
| Protective Action Criteria (PAC) | 18 [mg/m3]190 [mg/m3]1200 [mg/m3] |
| F | 3 |
| Wassergefährdungsklasse (WGK) | 3 |
| TSCA-Inventar-Status | TSCA listed |
| Zollcode | 29221980 |
| RTECS-Nr. | TY2900000 |
| R-Sätze (R) | 36/37/38 |
Anwendungen & Verwendung
Beschreibung
Abbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0.
Verwenden
Tris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.
Herstellung & Synthese
synthetische
The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.