Reaktionsgleichung
C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](N=C(N)N)[C@@H](O)[C@@H]2N=C(N)N)[C@H]([O:25][C@@H:26]2[O:31][C@@H:30]([CH2:32][OH:33])[C@H:29]([OH:34])[C@@H:28]([OH:35])[C@@H:27]2NC)[C@@]1(O)C=O.[OH:41]S(O)(=O)=O.N[C@H](C(O)=O)CCCCN>>[O:33]=[CH:32][C@@H:30]([C@H:29]([C@@H:28]([C@@H:27]([CH2:26][OH:25])[OH:41])[OH:35])[OH:34])[OH:31]Komponenten
Versuchsvorschrift
196ΔcadAΔldcCΔfadR/pCABD2 strain was cultured at 37° C. for 20 hours on the LB agar medium containing 20 mg/L of streptomycin sulfate. The cells on the agar medium were scraped, inoculated into 4 mL of an L-lysine production medium containing 20 mg/L of streptomycin sulfate contained in a large diameter test tube, and cultured at a culture temperature of 37° C. for 24 hours. For the main culture, the saccharified solution of Example 4 (1) prepared from starches produced by Chlorella kessleri (1.33 g/L in terms of glucose amount), and the hydrolysis reaction mixture of Example 4 (1) prepared from starches and fat or oil were used as carbon sources. As a control, the culture was performed in a medium containing glucose at the same concentration as a carbon source.
Aufarbeitung
- customcontained in a large diameter test tube
- waitcultured at a culture temperature of 37° C. for 24 hours
Quellen und Nachweise
- ORD-Eintrag
- ord-f4c6d5e1d5e24dd2a85fe6ea7fc86e16
- Quelldatensatz
- uspto-grants-2014_05
- Patentnummer
- US08728772B2
Verknüpfte Notizen
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