tert-Butyl methyl ether → HCID 9793812, Verbindung
Reaktionsgleichung
[CH:1]([CH:4]([CH:8]([CH3:10])[CH3:9])[C:5]([NH2:7])=[O:6])([CH3:3])C.[CH3:11]OC(C)(C)C>>[CH2:1]([C@@H:4]([CH:8]([CH3:9])[CH3:10])[C:5]([NH2:7])=[O:6])[CH2:3][CH3:11]Komponenten
3
Versuchsvorschrift
Assay of plasma samples: to test tubes containing 2 μg of the internal standard (diisopropyl acetamide), 0.5 ml of plasma (thawed at room temperature) and 5 ml of tert-butyl methyl ether (TBME) was added. The test tubes were vigorously vortexed for 30 sec and centrifuged at 3000 g for 10 minutes. The organic phase was separated and dried under reduced pressure using a vortex evaporator. Samples were then reconstituted with 150 μl of chloroform and dried under reduced pressure without vortexing. The sample was again reconstituted with 30 μl of chloroform, of which 2 μl were injected into the GC apparatus.
Aufarbeitung
- additionwas added
- waitcentrifuged at 3000 g for 10 minutes
- customThe organic phase was separated