Genite → Arachidonic Acid

HRID 1086417

Reaktionsgleichung

Reaktionsgleichung von HRID 1086417
[C:1]([O-:22])(=[O:21])[CH2:2][CH2:3][CH2:4]/[CH:5]=[CH:6]\[CH2:7]/[CH:8]=[CH:9]\[CH2:10]/[CH:11]=[CH:12]\[CH2:13]/[CH:14]=[CH:15]\[CH2:16][CH2:17][CH2:18][CH2:19][CH3:20].[Na+].C1C=CC(S(OC2C=CC(Cl)=CC=2Cl)(=O)=O)=CC=1.CC1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=O.CN1[C@H]2[C@@H](O)C[C@@H]1C[C@H](OC([C@@H](C1C=CC=CC=1)CO)=O)C2>>[C:1]([OH:22])(=[O:21])[CH2:2][CH2:3][CH2:4]/[CH:5]=[CH:6]\[CH2:7]/[CH:8]=[CH:9]\[CH2:10]/[CH:11]=[CH:12]\[CH2:13]/[CH:14]=[CH:15]\[CH2:16][CH2:17][CH2:18][CH2:19][CH3:20]

Komponenten

5

Edukt 4

HCID 7327Genite2×HCID 638015RetinalHCID 6918612Anisodamine2×HCID 236754535,8,11,14-Eicosatetraenoic acid, sodium salt, (all-Z)-3×

Produkt 1

HCID 444899Arachidonic Acid

Versuchsvorschrift

Finally, we investigated arachidonic acid-induced lipid peroxidation of the retinal suspension and any protection provided by the presence of anisodamine. Sodium arachidonate was freshly prepared with DPBS by ultrasonification. The experimental system consisted of 0.24 ml of the retinal suspension and, 0.03 ml anisodamine or DPBS for the control. To each tube, excluding the control, 0.03 ml of sodium arachidonate was added. The final concentration of sodium arachidonate was 0.25 mg/ml. The final concentration of anisodamine was 0.01 mg/ml. The malondialdehyde concentration was measured after incubation of the tubes at 37° C. for 30 minutes using the technique discussed previously.

Aufarbeitung

  1. customTo each tube, excluding
  2. concentrationThe malondialdehyde concentration
  3. customat 37° C.
  4. customfor 30 minutes

Quellen und Nachweise

ORD-Eintrag
ord-2677d0db2115458fb54a677295188ea9
Quelldatensatz
uspto-grants-1991_07
Patentnummer
US05030637

Verknüpfte Notizen

Wird geladen…