Indole-3-Acetic Acid → Indole-3-Acetic Acid

HRID 1164781

Reaktionsgleichung

Reaktionsgleichung von HRID 1164781
N1C2C(=CC=CC=2)C=C1CC(O)=O.[NH:14]1[C:22]2[C:17](=[CH:18][CH:19]=[CH:20][CH:21]=2)[C:16]([CH2:23][C:24]([OH:26])=[O:25])=[CH:15]1.C(N(CCCC)CCCC)CCC.C(OC(Cl)=O)C(C)C>CN(C)C=O.[OH-].[Na+]>[NH:14]1[C:22]2[C:17](=[CH:18][CH:19]=[CH:20][CH:21]=2)[C:16]([CH3:23])=[CH:15]1.[NH:14]1[C:22]2[C:17](=[CH:18][CH:19]=[CH:20][CH:21]=2)[C:16]([CH2:23][C:24]([OH:26])=[O:25])=[CH:15]1

Komponenten

8

Edukt 4

HCID 802Indole-3-Acetic AcidHCID 7622TributylamineHCID 62365Isobutyl chloroformateHCID 43717522-Indolylacetic acid

Produkt 2

HCID 802Indole-3-Acetic AcidHCID 6736Skatole

Reagenzien, Katalysatoren, Lösungsmittel 2

HCID 6228DimethylformamideLösungsmittel2×HCID 14798Sodium HydroxideLösungsmittel2×

Versuchsvorschrift

Anti-skatole antibodies were prepared essentially in the same manner as described by Weiler, Planta (1981) 153:319-324 for preparing antibodies to detect a plant hormone, indole acetic acid. A skatole analogue-, indole-3-acetic acid (IAA), bovine serum albumin (BSA) conjugate was prepared by a mixed anhydride method. IAA (52.3 mg, 300 μmol) in 2 ml dimethylformamide (DMF) was treated with 75 μl tri-n-butyl amine and the solution was cooled to about -10° to -15° C. Forty μl of isobutylchlorocarbonate was added to that mixture, and the solution was stirred for about 10 min. That mixture was continually stirred while a total of 420 mg of BSA, dissolved in a mixture of 23 ml 50% DMF solution and 420 μl of 1N NaOH was slowly added. Following addition of the BSA, the solution was stirred at 0° C. for an hour. 100 μl of 1N NaOH was then added. The mixture was stirred for an additional four hours at 0° C. That mixture was dialyzed twice against 2 L of 10% DMF and then against water (4×1 L).

Aufarbeitung

  1. customas described by Weiler, Planta (1981) 153:319-324 for preparing antibodies
  2. temperaturethe solution was cooled to about -10° to -15° C
  3. stirringThat mixture was continually stirred while a total of 420 mg of BSA
  4. additionwas slowly added
  5. additionaddition of the BSA
  6. stirringthe solution was stirred at 0° C. for an hour
  7. stirringThe mixture was stirred for an additional four hours at 0° C
  8. customThat mixture was dialyzed twice against 2 L of 10% DMF

Quellen und Nachweise

ORD-Eintrag
ord-52825fea3e5c4e9f97364452e4b99a4a
Quelldatensatz
uspto-grants-1990_03
Patentnummer
US04906563

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