Chloroform;3-methylbutan-1-ol;phenol → Phenol chloroform

HRID 1190782Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 1190782
[C:1]1([OH:7])[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1.[CH:8]([Cl:11])([Cl:10])[Cl:9].C(O)CC(C)C>C(Cl)(Cl)Cl.C(O)CC(C)C>[C:1]1([OH:7])[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1.[CH:8]([Cl:11])([Cl:10])[Cl:9]

Komponenten

3

Edukt 1

HCID 66587205Chloroform;3-methylbutan-1-ol;phenol

Produkt 1

HCID 18475210Phenol chloroform

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 16211727Chloroform Isoamyl AlcoholLösungsmittel

Versuchsvorschrift

To free DNA and/or RNA solutions from protein contaminations, the DNA or RNA solution was added to 1 vol. phenol/chloroform/isoamyl alcohol (25:24:1, v/v/v), mixed for 30 seconds and centrifuged for phase separation (13,000 rpm, 5 minutes, RT). The upper watery phase was subsequently transferred to a new reaction vessel and the procedure was repeated as many times as necessary so that after centrifugation, no protein phase was detected any more. Thereupon, for the removal of potentially present phenol residues—in a new reaction vessel—1 vol. chloroform/isoamyl alcohol (24:1, v/v) was added to the upper phase, mixed for 30 seconds and centrifuged again. Finally, the upper, DNA-containing phase was precipitated with ethanol.

Aufarbeitung

  1. additionmixed for 30 seconds and centrifuged for phase separation (13,000 rpm, 5 minutes, RT)
  2. customThe upper watery phase was subsequently transferred to a new reaction vessel
  3. customThereupon, for the removal of potentially present phenol
  4. customresidues—in a new reaction vessel—1
  5. additionmixed for 30 seconds
  6. customwas precipitated with ethanol

Quellen und Nachweise

ORD-Eintrag
ord-35fe0a51ceca4faca2d125112f0f7c8a
Quelldatensatz
uspto-grants-2014_03
Patentnummer
US08679799B2

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